Figure 3
From: The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules
Northern blot analysis of GcvB from Y. pestis strain KIM6. Total cell RNA was isolated from strain KIM6 grown in HIB at 30°C to an O.D.600 of 0.7 and probed with a 32P-labeled GcvB specific DNA probe as described in Methods. Two gcvB transcripts of about 206 and 130 nucleotides identified are indicated with arrows. Their sizes were determined based on their mobilities relative to the mobility of the E. coli GcvB RNA and 5S rRNAs (not shown).