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Table 5 VNTR locus-specific primers and the predicted sizes of amplicons from N. meningitidis strains Z2491, MC58 and FAM18.

From: Use of a multilocus variable-number tandem repeat analysis method for molecular subtyping and phylogenetic analysis of Neisseria meningitidis isolates

Locus

Primer designation

Primer sequence (5' → 3')a

Tm(°C)

Predicted size of amplicon (bp)

    

Z2491

MC58

FAM18

NMTR1

NMTR-1 F

6-FAM-GGGTCAAAAGACGGAAGTGA

54.9

351

421

379

 

NMTR-1 R

AAAATCATCCGAATCAATAAAGAC

49.8

   

NMTR2

NMTR-2 F

PET-GTGCGCCAGTAAGAAAATACAAT

53.9

327

236

341

 

NMTR-2 R

TCAGAAAAGTTTTGCATTTTGAA

50.1

   

NMTR6

NMTR-6 F

6-FAM-GCGGCATCTTTCATTTTGTC

52.8

165

165

207

 

NMTR-6 R

CGAAGAAGCGAAAGACCAAG

53.9

   

NMTR7

NMTR-7 F

CCATCCTTATCCGAATCTGAA

55

231

227

215

 

NMTR-7 R

VIC-CTGAAACCCTGCCTGAAGAA

53.4

   

NMTR9

NMTR-9 F

PET-GGAAAGAATGATGAAAATCAAAGC

51.3

243

225

396

 

NMTR-9 R

CCGTCTGAAAAGCGGATACC

55.8

   

NMTR9a

NMTR-9a F

GTTGTTGCCGACCAAGTTTT

54.4

218

188

248

 

NMTR-9a R

6-FAM-GAACCTTGCAATGCGTTCAC

55.2

   

NMTR9b

NMTR-9b F

CGACTTCATCGTCCACAAAA

53

200

200

182

 

NMTR-9b R

VIC-GGCTTTGTCTGCCTGTACG

56.3

   

NMTR9c

NMTR-9c F

GGAAATCTGCGCTTTCGTAG

54

202

187

202

 

NMTR-9c R

NED-TCATGTCAGCAATTCCCTCA

54

   

NMTR10

NMTR-10 F

NED-GGCATCGATGATGTGAAACA

53.3

221

251

221

 

NMTR-10 R

GTGCTGAAGCACCAAGTGAA

55.9

   

NMTR12

NMTR-12 F

CAAAGAGAGAGTGGAAGAACATCA

54.5

218

548

481

 

NMTR-12 R

PET-AATGACGAAGAGTGGCAGGATT

56.6

   

NMTR18

NMTR-18 F

AACGGAAAATTCCTGCACAA

53.1

182

182

209

 

NMTR-18 R

VIC-CGTTTTCCGTGTTCCTGATT

53.4

   

NMTR19

NMTR-19 F

NED-GACATATTGTGCGATGTCGAG

53.3

186

186

225

 

NMTR-19 R

CGCCAACAGAAAAGAATACGA

53.6

   
  1. a 6-FAM, VIC, NED and PET are ABI compatible dyes (Applied BioSystems, Foster City, CA, USA).