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Table 4 Specific argininosuccinate lyase (ASL) activity in exponential culture of WT, rpoS-, and ΔargR strains. Cultures were grown in M9 minimal media supplemented with 30 μM L-arginine and harvested at OD600 nm = 0.3. Cell extracts were assayed as described in the Methods section. ASL enzyme assay values were corrected for the low background levels of non-specific activity in the argH- mutant.

From: Stationary phase expression of the arginine biosynthetic operon argCBH in Escherichia coli

Strain

Genotype

Argininosuccinate lyase (U/mg protein)

Per cent of wild type

GC4468

WT

6.50 ± 0.083

100

GC122

rpoS -

1.53 ± 0.86

24

HS2404

ΔargR

159.6 ± 22.0

2,455

HS1072

argH-lacZ

0

0