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Table 3 Effect of ΔargR on culture growth in minimal media. Overnight cultures in LB were washed in minimal salts 3 times and subcultured at 1:1000 dilution into minimal media and grown at 37°C, 200 rpm, and samples were plated on minimal media to determine the average colony size.

From: Stationary phase expression of the arginine biosynthetic operon argCBH in Escherichia coli

Strain

Genotype

Media

Generation Time (h)

Average Colony (mm)

HS1066

argR +

M9

1.31 ± 0.12

1.21 ± 0.02

HS2404

ΔargR

M9

2.14 ± 0.28

0.74 ± 0.02

HS1066

argR +

M9 + P

1.21 ± 0.07

1.30 ± 0.03

HS2404

ΔargR

M9 + P

1.27 ± 0.23

0.73 ± 0.02

  1. P, Pyrimidine- Cytosine, Uracil and Thymine added at 20 μg per ml