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Table 1 Expression of the argCBH operon in rich media. Wildtype, ΔrpoS and ΔargR strains harboring the rsd1066 operon lacZ-fusion to the argCBH operon were grown in LB media and assayed for β-galactosidase activity in exponential phase (OD600 = 0.3) and stationary phase (OD600 = 1.5) as described in Methods. All reported activities are the average of three independent determinations.

From: Stationary phase expression of the arginine biosynthetic operon argCBH in Escherichia coli

Strain

Genotype

β-Galactosidase (Miller Units)

  

Exponential

Stationary

HS1066

WT

5.6 ± 0.34

38.4 ± 0.97

HS1066p

ΔrpoS

6.1 ± 0.26

18.8 ± 0.15

HS2404

ΔargR

469 ± 18

553 ± 33

HS2405

ΔrpoS ΔargR

486 ± 32

712 ± 35