Identification of potential CepR regulated genes using a cep box motif-based search of the Burkholderia cenocepacia genome

Table 1 OHL responsive genes identified by Tn5-OT182 mutagenesis of K56-I2

Transposon Mutant	Orf^a	Predicted start codon^b	Location of insert^b	Gene/domain homology^c	OHL effect on expression^d
K56-I2-P1, K56-I2-P2	BCAM0392	2:445357	2:444971	COG1835: Predicted acyltransferases	+
K56-I2-P3	BCAM0957	2:1062298	2:1060868	scpB: serine-carboxyl proteinase precursor	+
K56-I2-P5, K56-I2-P10	BCAS0293	3:328037	3:328810	aidA	+
K56-I2-P9	BCAM2631	2:2981279	2:2980753	COG2860: predicted membrane protein	-
K56-I2-P12	BCAM2630	2:2979794	2:2980345	phuV: hemin specific ATP-binding protein	+
K56-I2-2PB2	no gene		2:2980336		-
K56-I2-NB12	BCAM1187	2:1298085	2:1297891	COG4774, Outer membrane receptor	-

^a Open reading frame designation from the unpublished annotation of the B. cenocepacia J2315 genome.
^b Locations reported as chromosome:nucleotide.
^c Gene and domain homologies determined using BLASTP.
^d Effect of OHL on expression of the lacZ fusion created by insertion of the transposon. +, positive regulation or greater expression in the presence of OHL; -, negative regulation or lower expression in the presence of OHL.

ISSN: 1471-2180