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Table 3 LSMean values with Standard errors of the TIMP1 gene transcripts. Depicts the LSMean values with Standard errors of the TIMP1 gene transcripts expressed by macrophages over time and measured by Real Time RT PCR as described in materials and methods.

From: Cytokine responses of bovine macrophages to diverse clinical Mycobacterium avium subspecies paratuberculosis strains

Strain

Time post infection

 

2 hour

16 hour

24 hour

48 hour

96 hour

B1018

6.45 ± 0.28

16.65 ± 8.57

6.2 ± 0.09

11.63 ± 1.42

45.9 ± 8.74

S7565

12.4 ± 1.88

2 ± 0.67

5.74 ± 1.9

9.26 ± 0.5

25.2 ± 0.25

Hu6

3.12 ± 0.79

0.87 ± 0.16

1.1 ± 0.34

2.3 ± 0.3

34.6 ± 5.7

M. avium

2.62 ± 0.18

3.2 ± 1.5

3.5 ± 0.07

4.2 ± 0.36

6.4 ± 1.25

PC1x

6.1 ± 0.6

3.8 ± 0.04

10.7 ± 1.52

36.4 ± 3.39

159.7 ± 1.57

PC2y

6.3 ± 0.34

24 ± 1.53

2.2 ± 0.65

52.3 ± 6.91

38.6 ± 5.1

NCz

6.2 ± 0.91

26.6 ± 3.91

49.8 ± 23.26

584.2 ± 71.4

8.1 ± 3.46

  1. x indicates positive control 1 (PC1) – cells stimulated with LPS
  2. y indicates positive control 2 (PC2) – cells stimulated with LPS in conjunction with recombinant bovine Interferon gamma
  3. z indicates negative control (NC) – unstimulated cells