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Figure 6 | BMC Microbiology

Figure 6

From: Proteolytic cleavage of pertussis toxin S1 subunit is not essential for its activity in mammalian cells

Figure 6

ADP-ribosylation assay of mutant PT constructs. (A) Autoradiogram of cellular ADP-ribosylation assay after treatment of CHO cells with the indicated PT protein. The upper radiolabeled band (approximately 41 kDa) corresponds presumably with GiĪ±2 and GiĪ±3, while the lower band is PT-independent endogenously labeled band often seen in this assay with CHO cell lysates [9]. (B) Graphed data showing the mean (n = 3) cellular activity of the 2 deletion constructs that are not processed (activity of wild type PT was set at 100%) and the in vitro ADP-ribosylation activity of the same proteins. (C) Autoradiogram of cellular ADP-ribosylation assay after treatment of CHO cells with the indicated PT protein, demonstrating the retention of activity of the replacement mutant PT constructs.

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