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Figure 2 | BMC Microbiology

Figure 2

From: Surface expression, single-channel analysis and membrane topology of recombinant Chlamydia trachomatis Major Outer Membrane Protein

Figure 2

Expression and processing of C. trachomatis MOMP in BL21omp8 cells. Coomassie-stained 12% (w/v) SDS-PAGE of OM proteins extracted using 1%(w/v) OG (10 μg protein per lane) from cells induced for 4 hrs in LB medium, showing dependence of C. trachomatis MOMP expression and processing on temperature and leader sequence. Cells incubated at 16°C and 37°C were induced with 0.1 mM IPTG and 1 mM IPTG, respectively. omp8: non-transformed omp8 cells; oT-TR: cells expressing C. trachomatis MOMP with the OmpT leader; nL-TR: cells expressing C. trachomatis MOMP with its native leader.

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BMC Microbiology

ISSN: 1471-2180

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