Identification of a new family of enzymes with potential O-acetylpeptidoglycan esterase activity in both Gram-positive and Gram-negative bacteria

Table 1 Extent of peptidoglycan O-acetylation in bacteria possessing OAP gene clusters

Species	Strain	% O-Acetylation¹
Agrobacterium tumefaciens	A6	3.44 ± 0.79
	371	1.52 ± 0.36
Bacillus cereus	ATCC 10702	24.7 ± 0.09
Bacteroides fragilis	ATCC 25285	69.4 ± 1.2
Bacteroides thetaiotamicron	ATCC 29741	27.0 ± 1.2
Bradyrhizobium japonicum	532 C	18.5 ± 2.2
Campylobacter jejuni	ATCC 700819	62.7 ± 5.6
	NCTC 11168	55.7 ± 1.4
Chromobacterium violaceum	ATCC 12472	14.7 ± 2.5
Helicobacter pylori	ATCC 700392	45.6 ± 3.2
Photorhabdus luminescens	ATCC 29999	65.6 ± 1.6
Ruminococcus flavefaciens	007	33.4 ± 3.4

1. Average ± s.d.(n = 3)of base-labile acetate content relative to muramic acid concentrations in isolated and purified peptidoglycan.

ISSN: 1471-2180