From: The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803
Strain | Description | Reference/Source |
---|---|---|
Synechocystis 6803 | Glucose-tolerant 'wild-type' strain | [35] |
pta | ORF slr2132 inactivated by insertion of EmR gene into Pst I site at position 1,251,313 of the genome (872 bp into the coding seq of slr2132). | This study |
dspA | ORF sll0698 inactivated by insertion of KmR gene into Eco R1site at position 125,060 of the genome (1384 bp into the coding seq of sll0698). | This study |
ptadspA | ORF sll0698 inactivated in pta | This study |
pBluescriptII KS + | Multipurpose cloning vector with AmpR. | Stratagene |
pBSEmEco1 | Cloned EmR gene cassette. | [48,49] |
pUC4K | Cloned KmR gene cassette | Stratagene |