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Table 1 Bacterial strains and plasmids used

From: The influence of acetyl phosphate on DspA signalling in the Cyanobacterium Synechocystis sp. PCC6803

Strain

Description

Reference/Source

Synechocystis 6803

Glucose-tolerant 'wild-type' strain

[35]

pta

ORF slr2132 inactivated by insertion of EmR gene into Pst I site at position 1,251,313 of the genome (872 bp into the coding seq of slr2132).

This study

dspA

ORF sll0698 inactivated by insertion of KmR gene into Eco R1site at position 125,060 of the genome (1384 bp into the coding seq of sll0698).

This study

ptadspA

ORF sll0698 inactivated in pta

This study

pBluescriptII KS +

Multipurpose cloning vector with AmpR.

Stratagene

pBSEmEco1

Cloned EmR gene cassette.

[48,49]

pUC4K

Cloned KmR gene cassette

Stratagene