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Table 4 H-Agglutination reaction of fliC-H4 recombinant plasmid carrying E. coli K-12 strains

From: Genetical and functional investigation of fliC genes encoding flagellar serotype H4 in wildtype strains of Escherichia coli and in a laboratory E. coli K-12 strain expressing flagellar antigen type H48

Strain

serotype or fliC recombinant JM109 derivative

agglutination with H-specific antiseraa

  

H48

H4U9-41

H4P12b

U9-41

O2:K1:H4

<200b

12800

12800

P12b

O15:H17

<200b

6400

12800

JM109c

O-rough:H48

12800

<200b

<200b

TPE1976

JM109 (pLITMUS38-fliC-H4U9-41)d

12800

12800

12800

TPE1978

JM109 (pLITMUS38-fliC-H4P12b)e

12800

6400

12800

  1. a) reciprocal value agglutination titers with antisera (the same results were obtained in two separate experiments). H4U9-41 and H4P12b indicates the H4 antisera produced with strains U9-41 and P12b, respectively.
  2. b) no agglutination with start serum dilution 1:200
  3. c) E. coli K-12 [23]. d) fliC-H4 gene cloned from strain U9-41
  4. e) fliC-H4 gene cloned from strain P12b