Genetical and functional investigation of fliC genes encoding flagellar serotype H4 in wildtype strains of Escherichia coli and in a laboratory E. coli K-12 strain expressing flagellar antigen type H48

Table 1 Agglutination titers with H4 antisera derived from strains U9-41 and P12b

Strain	reported serotype	agglutination with antisera^a
		H4_U9-41	H4_P12b
U9-41	O2:K1:H4^b	12800	12800
U1-41	O5:K4:H4^b	25600	25600
P7d	O68:H4^b	1600	3200
E1541-68	O154:H4^b	3200	1600
P12b	O15:H17^c,e	6400	12800
872-69	O20:H17^d,e	12800	12800
107-74	O15:H17^d,e	3200	3200
305-78	O15:H17^d,e	3200	3200
870-69	O20:H17^d,e	6400	12800
327-01	O77:H17^d,e	12800	12800

a) reciprocal value of agglutination titers with antisera (the same results were obtained in two separate experiments). H4_U9-41 and H4_P12b indicates the H4 antisera produced with strains U9-41 and P12b, respectively.
b) E. coli standard test strain [3-5]
c) E. coli standard test H-test strain [3-5], able to produce spontaneously a variant expressing flagellar antigen H4 and containing a fliC-H4 gene [21, 22]
d) strains and serotype data provided from Flemming Scheutz, Statens Seruminstitut, Copenhagen, Denmark
e) all H17 strains were shown to carry a fliC-H4 genotype and expressed flagellar H4 antigens (this work).

ISSN: 1471-2180