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Figure 4 | BMC Microbiology

Figure 4

From: Genetical and functional investigation of fliC genes encoding flagellar serotype H4 in wildtype strains of Escherichia coli and in a laboratory E. coli K-12 strain expressing flagellar antigen type H48

Figure 4

(A) Low power micrograph of E. coli strain TPE1978 cells showing the density of the bacterial samples used for indirect IEM and the presentation of flagella (bar length = 1 μm) (B) IEM of strain TPE1978 flagella after incubation with rabbit flagellar H48 antiserum (1:1000) and detection of bound antibody by anti-rabbit-IgG- 10 nm gold (1:20), bar length = 100 nm. (C) Strain JM109 flagella after incubation with rabbit flagellar H48 antiserum and detection of bound antibody by anti-rabbit-IgG- 10 nm gold. (D) Strain TPE1978 flagella after incubation with rabbit flagellar H4 antiserum (1:1000) and detection of bound antibody by anti-rabbit-IgG- 5 nm gold. (E) Strain JM109 flagella after incubation with rabbit flagellar H4 antiserum and detection of bound antibody by anti-rabbit-IgG- 5 nm gold. (F) Double-labeling IEM of strain TPE1978 after sequential incubations with rabbit flagellar H4 antiserum and anti-rabbit-IgG 5 nm gold, followed by rabbit flagellar H48 antiserum detected by anti-rabbit-IgG- 10 nm gold. Both, 5 nm and 10 nm gold markers are bound at comparable amounts over all flagella present on the bacteria. (G) Double-labeling IEM of strain JM109 after sequential incubations with rabbit flagellar H4 antiserum and anti-rabbit-IgG- 5 nm gold followed by rabbit flagellar H48 antiserum and anti-rabbit-IgG- 10 nm gold. Only H48 specific (10 nm) gold particles are bound to the flagella of JM109.

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