Figure 2

(A) shows a gel image of representative FAFLP patterns of English H. pylori strains with the bands in red indicating the internal lane standards (Genescan Rox 500). (B) Representative phylogenetic tree indicating genetic relationships between English H. pylori strains (n = 16) and those from other world populations (HupB, n = 3; Ire, n = 3; Italy (Sard), n = 2; R = 2; E. Asia (Hu), n = 2; India (L, MS, BJ), n = 5) based on the FAFLP profiles generated for the strains from different countries (described in the materials and methods section). The neighbour joining network tree created based on the level of similarity between the amplitypes shows that European strains form a distinct cluster although two other clusters showing associations with the Indian strains are also seen. (C) Cluster analysis of the 296 bp glm M was generated with 10 English strains, 2 strains each from India (L), E. Asia (Hu), Peru (Sjm) and S. Africa (R). 4 each from Ire and HupB and the sequenced HP26695. The rounded circle here shows the grouping of English islolate (N 17) with the Indian (L) and E. Asian (Hu) strains. (D) Tree constructed from the bab B gene products from different strains (N, n = 8; Ire, n = 4; HupB and MS, L, n = 3 respectively, 2 each of Hu and Sjm with HP26695). (E) Genetic relationships of the English strains based on the 250 bp sequence of the 5'end of the cag A gene (England, n = 7; East Asia, n = 4; S. Africa, n = 1; W. Africa, n = 2; S. America, n = 2; central America, n = 1; India, n = 2; Bangladesh, n = 2 and Holland, n = 1). A few strains from Nottingham showed sequence similarity with the Asian strains as indicated by the circles in green. (F) Phylogenetic tree based on the REP fingerprinting patterns generated by the English strains (n= 5) in comparison with 5 strains from Ire and 6 from India (L).