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Figure 6 | BMC Microbiology

Figure 6

From: An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants

Figure 6

Effect of deletions of P. aeruginosa GntR homologs on fabA expression. The indicated genes were deleted in strain PAO1 containing a chromosomally integrated fabA'-lacZ fusion (labelled PAO1). The control was PAO1 with a fabA Δ30'-lacZ fusion; this strain harbours the same fabA'-lacZ fusion but contains a deletion of the putative 30 nucleotide activator-binding site in the fabA promoter region. Strains were grown to mid-log phase in LB medium containing 0.05% Brij 58 +/- 0.05% oleic acid and β-galactosidase expression was monitored in triplicate samples. The dotted line marks expression levels observed in the putative activator binding-site mutant and similar levels would be expected in an activator mutant.

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