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Figure 1 | BMC Microbiology

Figure 1

From: An improved method for rapid generation of unmarked Pseudomonas aeruginosa deletion mutants

Figure 1

Map of the Gateway-compatible pEX18ApGW. This vector was derived by cloning a Gateway conversion fragment (grey) into the multiple cloning site of pEX18Ap (black). Only selected restriction enzyme cleavage sites are shown. Abbreviations: attR1 and attR2, bacteriophage λ recombination sites; bla, β-lactamase-encoding gene; cat, chloramphenicol acetyl transferase-encoding gene; ccdB, gene encoding gyrase-modifying enzyme (CcdB poisons host DNA gyrase by forming a covalent complex with the DNA gyrase A subunit and thus serves as a counter-selectable marker in gyrA+ cloning hosts [25]); ori, ColE1-derived replication origin; oriT, origin of conjugal transfer; sacB, Bacillus subtilis levansucrase-encoding gene. The sequence of this plasmid was deposited in GenBank and assigned accession number AY928469.

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