Gene | Primer | Oligonucleotide sequence (5'-3')c | Fragment size (bp) | Annealing temperature | Primer coordinates | Accession number | Reference |
---|---|---|---|---|---|---|---|
eae a | EAE-1 EAE-2 | GGAACGGCAGAGGTTAATCTGCAG GGCGCTCATCATAGTCTTTC | 775 | 55°C | 1441–1460 2193–2215 | AF022236 | 7 |
eae-α1 | EAE-FB EAE-A | AAAACCGCGGAGATGACTTC CACTCTTCGCATCTTGAGCT | 820 | 60°C | 1909–1928 2709–2728 | AF022236 | 8 |
eae-α2 | IH2498aF IH2498aR | AGACCTTAGGTACATTAAGTAAGC TCCTGAGAAGAGGGTAATC | 517 | 60°C | 2099–2122 2597–2615 | AF530555 | 8 |
eae-β1 | B1A B1B | ACTTCGCCACTTAATGCCAGC TTGCAGCACCCCATGTTGAAT | 730 | 66°C | 1924–1944 2633–2653 | AF453441 | 9 |
eae-ξR/β2Bb,e | B2A B2B | AAGGGGGGAACCCCTGTGTCA ATTTATTCGCAGCCCCCCACG | 604 | 66°C | 2056–2076 2639–2659 | AF530556 AJ715407 | 9 |
eae-δ/κ/β2Oc | EAE-FB EAE-D | AAAACCGCGGAGATGACTTC CTTGATACACCCGATGGTAAC | 833 | 60°C | 1909–1928 2721–2741 | U66102 | 8 |
eae-γ1 | EAE-FB EAE-C1 | AAAACCGCGGAGATGACTTC AGAACGCTGCTCACTAGATGTC | 804 | 60°C | 1909–1928 2691–2712 | AF071034 | 8 |
eae-θ/γ2d | EAE-FB EAE-C2 | AAAACCGCGGAGATGACTTC CTGATATTTTATCAGCTTCA | 808 | 58°C | 1909–1928 2697–2716 | AF025311 | 8 |
eae-ε1 | EAE-FB LP5 | AAAACCGCGGAGATGACTTC AGCTCACTCGTAGATGACGGCAAGCG | 722 | 66°C | 1909–1928 2605–2630 | AF116899 | 8 |
eae-νR/ε2e | EAE-E2F EAE-E2R | AATACAGAAGTTAAGGCAT ACGACCACTATTCATTTC | 378 | 58°C | 2230–2248 2590–2607 | AF530554 | 9 |
eae-ζ | Z1 Z2 | GGTAAGCCGTTATCTGCC ATAGCAAGTGGGGTGAAG | 206 | 62°C | 2062–2079 2250–2267 | AF449417 | 8 |
eae-η | EAE-FB ETA-B | AAAACCGCGGAGATGACTTC TAAGCGACCACTATTCGTG | 702 | 60°C | 1899–1918 2582–2600 | AJ308550 | 9 |
eae-ι1 | EAE-FB IOTA-B | AAAACCGCGGAGATGACTTC GTCATATTTAACTTTTACACTA | 651 | 55°C | 1909–1928 2538–2559 | AJ308551 | 9 |
eae-μR/ι2e | Iota2-F Iota2-R | CTGGTAAAGCGATAGTCAAAC GCGTTTTTGAAGAAACATTTTGC | 936 | 58°C | 1850–1870 2763–2785 | AF530553 | 9 |
eae-λ | 68.4F 68.4R | CGGTCAGCCTGTGAAGGGC ATAGATGCCTCTTCCGGTATT | 466 | 64°C | 2061–2079 2506–2526 | AJ715409 | 8 |
eae-μBf | EAE-FB FV373R | AAAACCGCGGAGATGACTTC ACTCATCATAATAAGCTTTTTGG | 665 | 60°C | 1909–1928 2541–2563 | AJ705049 | 9 |
eae-νBf | IH1229aF IH1229aR | CACAGCTTACAATTGATAACA CTCACTATAAGTCATACGACT | 311 | 60°C | 269–289 559–579 | AJ705050 | 8 |
eae-ξBf | EAE-FB B49R | AAAACCGCGGAGATGACTTC ACCACCTTTAGCAGTCAATTTG | 468 | 66°C | 1909–1928 363–384 | AF116899 AJ705051 | 8 |