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Table 4 Genetic relationship of the new intimin gene (η2) (AJ879898, AJ879899, AJ879900) detected in bovine EPEC strains of serotype ONT:H45 and the remaining eae variants: Pairwise alignments calculated with CLUSTAL W

From: Serotypes, intimin variants and other virulence factors of eae positive Escherichia coli strains isolated from healthy cattle in Switzerland. Identification of a new intimin variant gene (eae-η2)

Designation of intimins in this study

Designation of intimins in previous studies (References)

ORF length (bp)

Reference strain

Serotype

Origin

Accession number

Genetic relationship identities

α1

α (4) and α1 (5)

2820

E2348/69

O127:H6

Human

M58154

87%

α2

α (4) and α2 (5)

2820

E. coli

O125:H6

Human

AF530555

87%

β1

β (4) and β1 (8)

2820

RDEC1

O15:H-

Rabbit

AF200363

84%

ξR/β2B

ξ (14) and β2 (8)

2820

KB411

ONT:HNT

Bovine

AF530556

88%

ξR/β2B

ξ (14) and β2 (8)

2820

FV359

O119:H6

Human

AJ715407

88%

δ/β2O

δ (4) and β2 (5)

2820

BL152.1

O86:H34

Human

AJ875027

87%

κ

κ (6)

2820

6044/95

O118:H5

Human

AJ308552

87%

γ1

γ (4) and γ1(5)

2805

EDL933

O157:H7

Human

AF071034

86%

γ2/θ

γ2 (5) and θ (13)

2808

CL-37

O111:H8

Human

AF449418

84%

ε1

ε(5)

2847

PMK5

O103:H2

Human

AF116899

92%

νR/ε2

ν (14) and ε2 (GenBank AF530554)

2847

VR64/4

O2related:H19

Ovine

AF530554

94%

ζ

ζ (13)

2817

4795/95

O84:H4

Human

AJ271407

86%

η1

η (6)

2847

CF11201

O125:H-

Human

AJ308550

98%

η2

new (this study)

2847

H03/53199a

ONT:H45

Human

AJ876652

100%

ι1

ι (6)

2814

7476/96

O145:H4

Human

AJ308551

88%

μR/ι2

μ (14) and ι2 (GenBank AF530553)

2814

VR45

OR:H-

Ovine

AF530553

83%

λ

λ (8, 14)

2817

EPEC-68.4

O34:H-

Human

AJ715409

86%

μB

μ (8)

2808

EPEC-373

O55:H51

Human

AJ705049

87%

νB

ν (8)

2823

IH1229a

O10:H-

Human

AJ705050

82%

ξB

ξ (8)

2847

STEC-B49

O80:H-

Bovine

AJ705051

91%