Identification to the species level of Lactobacillus isolated in probiotic prospecting studies of human, animal or food origin by 16S-23S rRNA restriction profiling

Table 3 Patterns of restriction digestion of short 16S-23S intergenic spacer of lactobacilli and other bacteria isolated from different sources

Isolate	Sph I	Nco I	Nhe I	Ssp I	Sfu I	Eco RV	Dra I	Vsp I	Hinc II	Eco RI	Hind III	Identification
Chicken gastrointestinal tract
F 5.2 F 5.3	+	-	-	+	+	-	-	+	+	-	-	Lact. plantarum b
F 5.7	-	+	-	-	-	+	-	-	-	+	+	Lact. crispatus
Reg 24 25 26	-	-	-	-	-	+	-	-	-	-	-	Lact. johnsonii ^a
Reg 32	-	-	-	-	-	+	-	-	-	-	-	Lact. gasseri ^a
Reg 34	+	+	-	-	-	-	-	+	-	-	-	Lact. reuteri
38 90 110	+	+	-	-	-	-	-	+	-	-	+	Lact. vaginalis
98, 108	-	-	-	-	-	+	+	-	-	-	+	Lact. rhamnosus
115	-	+	-	-	-	-	-	-	-	+	+	Lact. acidophilus
'Coalho' cheese
AB2	-	-	-	-	-	+	+	+	-	-	+	Lact. casei
AB3	+	-	-	-	-	-	-	+	-	-	-	Lact. fermentum
AQ1	-	-	-	-	-	+	+	-	-	-	+	Lact. rhamnosus
LV3	-	+	-	-	-	-	-	-	-	+	+	Lact. acidophilus
CM3	-	-	-	+	-	-	+	-	-	-	+	W. confusa
AB1 BL1 SM4	-	-	-	+	-	-	-	-	-	-	-	Strep. thermophilus
CM2M17	-	-	-	-	-	-	+	-	-	-	-	L. lactis
AQ2 CM4 LV1	-	-	-	+	-	-	-	-	-	-	-	Ent. faecalis ^b
AQ2AT1	+	-	+	+	+	-	+	+	+	+	+	Staph. aureus
Cassava starch sour fermentation
P16.01 P25.01	+	-	-	-	-	-	-	+	-	-	-	Lact. fermentum
P24.03 P25.04	+	-	-	+	+	-	-	+	+	-	-	Lact. plantarum b
P24.01 P29.01	+	-	-	+	-	-	-	+	+	-	-	Lact. plantarum a
P10.06	+	-	-	-	-	-	-	+	-	-	+	Lact. brevis
P10.02	-	-	-	+	-	-	+	+	+	-	-	Lact. salivarius
P18.04	-	-	-	-	-	+	-	-	-	-	+	Lact. perolens
Human sources
UFV H2b20	-	+	-	-	+	-	-	-	-	-	-	Lact. delbrueckii
117	-	-	-	-	-	+	+	-	-	-	+	Lact. rhamnosus
A15 A91	-	-	-	+	+	-	+	-	-	-	-	Lact. ruminis
PM1	-	-	-	-	-	+	-	-	-	-	-	Lact. johnsonii ^a
PM2 PM3	-	-	-	-	-	+	-	-	-	-	-	Lact. jensenii ^a

^aSpecies identified by additional restriction digestion with Hind III, Bgl II and Pvu II of long spacer regions
^bSpecies identified by amplicons sizes

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