A genomic island present along the bacterial chromosome of the Parachlamydiaceae UWE25, an obligate amoebal endosymbiont, encodes a potentially functional F-like conjugative DNA transfer system

Table 1 Description of main features of the parachlamydial 100-kb genomic island. Chromosome location of direct repeats, tRNA genes, tra operon, transposases, bacteriophage-related proteins and proteins involved in DNA metabolism is listed below.

	Protein number^a	Position^a
Direct repeat	-	1648147–1648157
gly-tRNA ^a,b	-	1648172–1648243
gly-tRNA ^a,b	-	1648332–1648403
Transposase^c	pc1402	1679924–1680400
Phage-related protein^c	pc1404	1681569–1682441
Putative transcriptional regulator^c,d	pc1405	1679924–1680400
Phage-related protein^c	pc1410	1685329–1686447
Putative transposase^c	pc1419	1695418–1696245
tra operon^e	pc1420-1441	1696410–1716241
Transposases^e	pc1426-1427	1700887–1701896
Putative DNA-binding protein^c	pc1443	1716648–1717004
Phage-related protein^c	pc1444	1717137–1717400
Direct repeat	-	1723093–1723103
Putative ATPase involved in DNA repair^c	pc1451	1723169–1723504
Probable Doc (death on cure) protein, bacteriophage P1^a	pc1456	1732622–1732999
Putative DNA-binding protein	pc1461	1735745–1736065
Putative transposase^c	pc1465	1740371–1741198
Probable DNA double-strand break repair ATPase	pc1467	1742079–1744181
Putative transposase	pc1468	1744634–1745023
Probable resolvase^a	pc1469	1745398–1745955
Probable transposases, partial length^a	pc1470-1471	1745807–1746692
Probable Doc (death on cure) protein, bacteriophage P1^a	pc1473	1747135–1747512
Phage-related protein^c	pc1474	1747618–1747809
Direct repeat	-	1747915–1747925

^a, according to Horn et al. [14];
^b, positive strand (cooriented as the majority of the genes of the GI); gly-tRNA s are separated by 88 nt;
^c, identified by BLAST [35; 36] and CLUSTALW [39] by ourselves;
^d, phage-related protein based on additional BLAST hit;
^e, partially annotated by Horn et al. [14] and further characterized by ourselves by BLAST [35, 36].

ISSN: 1471-2180