Development of real-time PCR for detection of Mycoplasma hominis

BMC Microbiology

Table 3 Reproducibility of the LightCycler-PCR

Copy number	No. of PCR runs where fluorescence gave positive signal	CP	SD	CV % of CP
10⁵	10 / 10	25.4	1.7	6.7
10⁴	10 / 10	28.7	1.6	5.5
10³	10 / 10	32.3	1.6	4.8
10²	10 / 10	35.8	1.7	4.8
10¹	10 / 10	39.7	2.1	5.3
5 copies	10 / 10	40.6	2.3	5.6
1 copy	6 / 10	43.8	1.7	3.8

CP = crossing-points, mean value
CV = coefficient of variation
SD = standard deviation
Statistical analysis of standard dilution series of standard PG21 DNA was performed. Ten different runs were analysed for crossing-points values and for presence of fluorescence signal. Based on crossing-points values the standard deviation of single concentration was calculated and coefficient of variation as a function: CV% = (SD / Average CP value for each concentration) × 100%.

ISSN: 1471-2180