- Research article
- Open Access
Yersinia enterocolitica type III secretion: Evidence for the ability to transport proteins that are folded prior to secretion
© Wilharm et al; licensee BioMed Central Ltd. 2004
Received: 10 December 2003
Accepted: 12 July 2004
Published: 12 July 2004
Pathogenic Yersinia species (Y. enterocolitica, Y. pestis, Y. pseudotuberculosis) share a t ype t hree s ecretion s ystem (TTSS) which allows translocation of effector proteins (called Yops) into host cells. It is believed that proteins are delivered through a hollow needle with an inner diameter of 2–3 nm. Thus transport seems to require substrates which are essentially unfolded. Recent work from different groups suggests that the Yersinia TTSS cannot accommodate substrates which are folded prior to secretion. It was suggested that folding is prevented either by co-translational secretion or by the assistance of s pecific Y op c haperones (called Sycs).
In this study we have fused YopE secretion signals of various length to the mouse dihydrofolate reductase (DHFR) in order to analyse the DHFR folding state prior to secretion. We could demonstrate that secretion-deficient as well as secretion-competent YopE-DHFR fusions complexed to SycE can be efficiently purified from Yersinia cytosol by affinity chromatography using methotrexate-agarose. This implies the folding of the DHFR fusion moiety despite SycE binding and contradicts the previously presented model of folding inhibition by chaperone binding. Secretion-deficient YopE-DHFR fusions caused severe jamming of the TTSS. This observation contradicts the co-translational secretion model.
We present evidence that the Yersinia TTSS is familiar with the processing of transport substrates which are folded prior to secretion. We therefore predict that an unfoldase is involved in type III secretion.
Pathogenic Yersinia species use a type III secretion system (TTSS) encoded by the virulence plasmid to inject a number of effector proteins (Y ersinia o uter p roteins, abbrev. Yops) into eucaryotic host cells [1–3]. The complex transport machinery (also denoted as injectisome) consists of some 25 components. A needle-like structure, polymerized from YscF, with a hollow center of about 2–3 nm seems to form a conduit for Yop transport into eucaryotic host cells .
The transport signal of Yops has been assigned to the first 10 to 17 codons of the respective gene [5–8]. Yet, what makes the signal a signal is not clear. Anderson and Schneewind  proposed a signal at the mRNA-level. This mRNA element was suggested to couple translation and secretion of Yops, implying a co-translational transport mode . Ramamurthi and Schneewind [9, 10] recently presented further evidence that the YopQ secretion signal might not be proteinaceous. However, work from Lloyd et al. [11, 12] supports the idea of an N-terminal peptide moiety with characteristics of an amphipathic sequence acting as transport signal for YopE.
As controversial as the question of the signal's nature is that of the mode of transport. The mRNA signal hypothesis is linked to a co-translational secretion model [5, 6], whereas an N-terminal signal peptide is compatible with both a co- or post-translational transport mechanism. This issue is even more complicated by the fact that the transport of several Yops is assisted by their s pecific Y op c haperones (e.g. YopE is assisted by SycE) whereas others such as YopQ – as far as known – are not assisted by chaperones. The minimal binding site of SycE has been mapped to residues 23–50 of YopE [7, 13, 14]. This binding site in complex with a SycE dimer has been suggested to constitute an additional, three-dimensional secretion signal  introducing a hierarchy of effector targeting . SycE binding has been shown to be dispensable for secretion of YopE to the culture supernatant but is required for translocation into eucaryotic cells [11, 16]. Additionally, YopE can be targeted to the type III machinery and secreted to the supernatant, although less efficient, without the first 15 N-terminal codons/aa residues solely by means of SycE assistance . Post-translational secretion of YopE has been demonstrated [6, 11, 18].
With the evidence for a post-translational secretion mechanism the question arose whether Yop transport occurs in a (partially) folded or unfolded form. Mouse dihydrofolate reductase (DHFR; 21.6 kDa) has been successfully applied as a reporter in eucaryotic systems to address the question of the folding state of transported proteins [19–22]. The stability of the folded DHFR domain can be manipulated by treatment with specific ligands such as methotrexate (MTX).
Recently, two studies were published applying the DHFR tool to analyse the properties of the Yersinia TTSS. Lee and Schneewind  reported on a YopE1–15-DHFR fusion and a YopE1–220-DHFR fusion (full length of YopE) which were not secreted by the TTSS of Yersinia enterocolitica. This lack of secretion competence however did not interfere with secretion of other Yops. Further, ubiquitin which rapidly folds into a stable tertiary structure was fused to YopQ and YopE signals of different length. Again, all fusions were secretion-incompetent and did not interfere with secretion of other Yops. Only when a folding-deficient ubiquitin was fused to the YopE1–15 signal was efficient secretion observed. Thus, it was concluded by Lee and Schneewind  that folding of DHFR and ubiquitin hindered secretion of the fusions. Further, the observation that the secretion-incompetent proteins did not lead to an occlusion of the TTSS prompted the authors to propose a model supporting the mRNA signal hypothesis: After signal recognition a substrate that cannot be accommodated would be rejected and – after separation from its mRNA – could not be reinitiated into the secretion machinery.
A second report deals with the role of SycE in secretion of YopE-DHFR fusions . DHFR and folding deficient mutants thereof were fused to YopE signals encompassing the first 16, 52, 80 and all 219 amino acid (aa) residues. The major outcome of this study was that wild-type DHFR could only be secreted when fused to YopE1–52 and that this secretion was strictly dependent on the presence of SycE. All other fusions required mutations in DHFR in order to allow secretion. Altogether, Feldman et al.  concluded that folding of DHFR was not compatible with its secretion competence and that SycE allowed secretion of wild-type DHFR as part of the YopE1–52 fusion by preventing its folding. In apparent contrast to Lee and Schneewind , Feldman et al.  could demonstrate the occlusion of the TTSS by several YopE-DHFR fusion constructs.
In parallel to these groups we have also applied the DHFR approach to the Yersinia enterocolitica TTSS. Contrary to the studies mentioned above our conclusion is that the Yersinia TTSS can deal with secretion substrates essentially folded prior to secretion. Since the transport channel seems to be too narrow to allow passage of such folded domains we propose an unfoldase activity associated with the TTSS.
Results and discussion
We have noticed that for analysis of cytosolic fusions it was crucial to prepare spheroplasts. Initially, we had lysed complete yersiniae pretreated with these drugs and even though we had extensively washed the bacteria prior to lysis we found the complete pool of DHFR saturated with the ligands. Thus, either the periplasmic space was loaded with MTX/APT or the bacterial surface was coated so that after cell lysis the released ligands could bind to DHFR causing artificial protection towards thermolysin.
From the protease protection analyses of spheroplast preparations we can conclude the following. First, neither APT nor MTX efficiently enter the cytoplasm which limits the potential of the DHFR tool in Yersinia. Second, cytosolic YopE1–53-DHFR prepared from spheroplasts can be stabilised by addition of MTX or APT suggesting that the DHFR moiety is properly folded.
The latter conclusion was further confirmed by native gel electrophoresis of cytosolic preparations as represented in Fig. 2. We found that the electrophoretic mobility of YopE1–53-DHFR was significantly increased in the presence of MTX. This might be explained by a more rigid conformation of the DHFR-ligand complex. This finding shows that the complete pool of soluble YopE1–53-DHFR is accessible to MTX binding which suggests the folding of DHFR.
We could not rule out that the obvious discrepancy between our observations and the well supported hypothesis presented by Feldman et al. was due to the slight difference between our YopE1–53-DHFR construct and the YopE1–52-DHFR fusion studied by Feldman et al. . Thus, we mimicked their YopE1–52-DHFR construct to analyse a fusion with the identical aa sequence.
In summary, our data show show that the DHFR moiety of YopE1–52-DHFR and YopE1–53-DHFR fusions was predominantly folded in a state that was competent to bind MTX and that this folding state of DHFR was compatible with SycE binding to fusions YopE1–52-DHFR and YopE1–53-DHFR. Since these fusions were secretable, we conclude that folding of substrate proteins prior to secretion is compatible with Yersinia type III secretion in principle. Though in obvious discrepancy to the view of Lee and Schneewind  as well as that of Feldman et al.  we feel that our conclusions are well supported by several studies.
Feldman et al. have claimed that SycE bound to a YopE1–52-DHFR fusion prevents DHFR folding by overlapping the DHFR moiety physically . Since the crystal structure of the SycE dimer complexed to YopE23–78 clearly shows that YopE is wrapped around the SycE dimer  and not vice versa it is hard to imagine how SycE binding should overlap with the fused DHFR domain. Further, it is clear from the studies of Birtalan et al.  that the influence of SycE on YopE is restricted to the chaperone binding site and does not extend globally since the RhoGAP domain of a (SycE)2/YopE complex was catalytically active. Moreover, comparison of the structure of the SycE dimer uncomplexed  with that of SycE complexed to the YopE chaperone binding site  reveals that SycE serves as a purely static binding platform. Thus, an unfolding activity of SycE is unlikely. Hence, the Yersinia TTSS generally has to deal with folded secretion substrates. In addition, it has been shown by isothermal titration calorimetry studies of Tir/CesT from enteropathogenic E. coli as well as by enzymatic activity profiling of Salmonella enterica SigD/SigE  that these effectors are not globally unfolded when bound to their specific chaperones. This suggests that storage of for the most part folded effectors in the presence of their specific chaperones is a common feature of type III secretion systems. Stored effectors must be either unfolded prior to secretion or the TTSS must be able to secrete folded proteins. Recently, needles of the injectisomes of Shigella  and Yersinia TTSSs  have been visualized by electron microscopy showing a hollow center with a diameter of 20–30 Å. Needles of the Salmonella TTSS1 with an outer diameter of 13 Å are even thinner . In comparison, the minimal diameter of the YopE catalytic domain is approximately 25 Å , that of the YopH catalytic domain is at least 40 Å , and that of the heterologous substrate DHFR is around 32 Å . Ubiquitin, which cannot be secreted by the Yersinia TTSS  has a minimal diameter of approximately 24 Å . Thus, it is generally believed that secretion does not occur with substrates in a folded form. Therefore, we propose that TTSS systems possess unfolding machineries to process the stored pool of effectors. Accordingly, it seems possible that the machinery can unfold heterologous substrates under certain conditions. Substrates such as the fusions YopE1–18-DHFR and YopE1–138-DHFR are identified as potential secretion substrates and engaged by the machinery. Yet, due to the inability to unfold these proteins they cause plugging of the pore. Other fusions such as YopE1–52-DHFR and YopE1–53-DHFR can be unfolded with some efficiency. An explanation for the dependency of SycE to transport DHFR fusions might be that SycE recruits and facilitates the binding of the proposed unfoldase so that it can efficiently pull on the polypeptide chain and trap local unfolding fluctuations. As reviewed by Matouschek  the characteristics of a protein to resist unravelling are not determined by its stability against global unfolding. The resistance is rather determined by the local structure first encountered by the unfoldase after recognition of the substrate. In that light, small differences among fusion constructs may influence efficiency of unfoldase to unravel substrates and may lead to dramatically different secretion phenotypes.
Gorbalenya and Koonin  were the first to describe the relationship between Rho helicase, V- and F-type ATPases, and the flagellar ATPase FliI, an homologue of the Yersinia TTSS ATPase YscN. A recent phylogenetic classification of P-loop NTPases suggests that all these NTPases belong to the group of unfoldases . Thus, we hypothesise that the ATPase YscN could be a suitable candidate for unfolding of TTSS substrates prior to secretion.
We want to stress that this issue is far from being clarified and introduce to this discussion the possibility that unfolding of TTSS substrates prior to secretion may occur.
The folding state of type three secretion substrates before and during transport has not been clearly defined. Using a DHFR fusion approach we have addressed these questions. We found that DHFR ligands methotrexate and aminopterin poorly entered Yersinia enterocolitica cells which limited the applicability of the DHFR tool. YopE1–18-DHFR and YopE1–138-DHFR fusions proved secretion-deficient and caused plugging of the secretion machinery. This indicates that folding of these artificial secretion substrates interfered with the secretion process. However, we could demonstrate secretion of YopE1–52-DHFR and YopE1–53-DHFR fusions even after inhibition of protein synthesis. Further, we showed that folding of the DHFR-moiety of these fusions as monitored by ligand binding was compatible with binding of the YopE secretion chaperone SycE. We conclude that the Yersinia TTSS is familiar with the processing of transport substrates which are folded prior to secretion and postulate that an unfoldase is involved in type III secretion.
YopE-DHFR fusion constructs were generated by introducing a BamHI-SalI fragment of murine dhfr into vectors pCJYE18-G3, pCJYE53-G3 and pCJYE138-G3  after excission of the BamHI-SalI fragment encoding GFP3. The HindIII-BamHI fragment encoding the YopE amino-terminus of the respective YopE fusion also encodes SycE allowing the coexpression of this chaperone . The BamHI-SalI fragment encoding DHFR was generated by PCR using primers 5'-GGATCCATGGTTCGACCATTGAACTGC-3' and 5'-GTCGACGGCCGGATCTAAAGCCAGC-3'. The murine dhfr used as template was a gift from Walter Neupert. Resulting plasmids were termed pE18-DHFR, pE53-DHFR and pE138-DHFR. Plasmid pE52-DHFR encoding a YopE1–52-DHFR fusion was generated by HindIII-BamHI mediated excision of the sycE-yopE1–18 fragment from plasmid pE18-DHFR and insertion of a PCR-generated sycE-yopE1–52 fragment flanked by HindIII and BglII restriction sites.
Protein secretion and analysis
As host strains for expression and secretion of Yop-DHFR fusions served the Yersinia enterocolitica strain WA-314  harbouring the pYV virulence plasmid and its secretion deficient yscV/lcrD mutant WA-C (pYV-515) . Chloramphenicol (20 μg/ml), nalidixic acid (60 μg/ml), kanamycin (50 μg/ml) were used as selective antibiotics. Overnight cultures were incubated at 27°C in BHI medium. For secretion experiments yersiniae were diluted 1:20 from overnight cultures and incubated for 2 h at 37°C in BHI medium; if indicated, CaCl2 was added to 2.5 mM to completely suppress Yop secretion. For secretion of Yops BHI medium was supplemented with 10 mM MgCl2, 0.2% glucose and 5 mM EGTA (BHI-EGTA; ). Methotrexate (MTX) and aminopterin (APT), were dissolved in DMSO (100 mM stock concentration) and added to the medium 10 min before induction of Yop secretion to a final concentration of 1 mM. Accordingly, controls were treated with 1% DMSO. DMSO did not significantly influence secretion or viability of yersiniae cultures. Cells and supernatants were separated by centrifugation; proteins from supernatants were precipitated with TCA, the pellet washed twice with acetone, once with water. Cell pellets and protein pellets after TCA precipitation were resuspended in SDS-PAGE loading buffer as recently described  und subjected to SDS-PAGE and Western blotting. Yop-DHFR fusions were detected with monoclonal anti-DHFR antibodies (Becton Dickinson), SycE was detected with a polyclonal antiserum from rabbit. Specifics of each experiment are given in the figure legends.
For the analysis of protein secretion after inhibition of protein synthesis the standard protocol was modified as follows. Overnight cultures were diluted as described above and grown in the presence of 2.5 mM CaCl2 at 37°C for 2 h. Then tetracycline was added to a final concentration of 50 μg/ml to inhibit protein biosynthesis. After 15 min of continued incubation in the presence of tetracycline bacteria were pelleted and resuspended in BHI supplemented with EGTA and tetracycline. Bacteria were cultured for another 20 min and supernatant and cell pellets were analysed by western blotting as described above. The efficiency of tetracycline treatment was verified by a complete inhibition of cell growth and by comparison to recently published methods based on treatment with chloramphenicol [11, 18].
YopE-DHFR fusions were purified from yersiniae lysates using MTX-agarose (Sigma). Yersiniae expressing DHFR fusions were lysed by French press treatment and soluble supernatants after centrifugation were incubated with MTX-agarose beads on ice for 30 min. Beads were washed with PBS five times, subsequently resuspended in SDS loading buffer and subjected to SDS-PAGE.
Spheroplasts from yersiniae induced for Yop expression and treated with 1 mM methotrexate (MTX), 1 mM aminopterin (APT) as described above or untreated, respectively, were prepared as described . Speroplasts were washed three times and subsequently lysed by osmotic shock resuspending them in 10 mM Tris-HCl pH 7.5, and soluble supernatants after centrifugation (cytosolic fractions) were treated with thermolysin (0, 1, 10 μg/ml).
Native gel electrophoresis
6% AA gels (25 mM HEPES pH 7.4 (Invitrogen), 5 mM NaCl, 1 mM DTT) were pre-run for 30 min at 80 V prior to sample application . Samples were loaded using a 5× sample buffer (25 mM HEPES pH 7.4, 5 mM NaCl, 1 mM DTT, 50% glycerole) and subjected to electrophoresis at 80 V for 2–3 h. Subsequently gels were electroblotted. Cytosolic fractions were prepared from spheroplasts after osmotic shock lysis and concentrated on Amicon (Millipore) concentrators (10 kDa molecular weight cut off) if necessary. Protein concentration was determined (Bio-Rad, Protein Assay) and 5 μg of protein was loaded per lane. MTX (100 mM stock solution, dissolved in DMSO) was added to 1 mM final concentration (to controls 1% of DMSO was added).
We are grateful to Walter Neupert for providing dhfr template. We thank Christoph Jacobi for providing plasmids pCJYE18-G3, pCJYE53-G3 and pCJYE138-G3 and Konrad Trülzsch and Susanne Kähler for critical reading of the manuscript. This work was supported by the Deutsche Forschungsgemeinschaft (SFB 594 Teilprojekt B6).
- Rosqvist R, Magnusson KE, Wolf-Watz H: Target cell contact triggers expression and polarized transfer of Yersinia YopE cytotoxin into mammalian cells. EMBO J. 1994, 13: 964-972.PubMed CentralPubMedGoogle Scholar
- Cornelis GR: The Yersinia Ysc-Yop virulence apparatus. Int J Med Microbiol. 2002, 291: 455-462.View ArticlePubMedGoogle Scholar
- Cornelis GR, Boland A, Boyd AP, Geuijen C, Iriarte M, Neyt C, Sory MP, Stainier I: The virulence plasmid of Yersinia, an antihost genome. Microbiol Mol Biol Rev. 1998, 62: 1315-1352.PubMed CentralPubMedGoogle Scholar
- Hoiczyk E, Blobel G: Polymerization of a single protein of the pathogen Yersinia enterocolitica into needles punctures eukaryotic cells. Proc Natl Acad Sci USA. 2001, 98: 4669-4674. 10.1073/pnas.071065798.PubMed CentralView ArticlePubMedGoogle Scholar
- Anderson DM, Schneewind O: A mRNA signal for the type III secretion of Yop proteins by Yersinia enterocolitica. Science. 1997, 278: 1140-1143. 10.1126/science.278.5340.1140.View ArticlePubMedGoogle Scholar
- Anderson DM, Schneewind O: Yersinia enterocolitica type III secretion: an mRNA signal that couples translation and secretion of YopQ. Mol Microbiol. 1999, 31: 1139-1148. 10.1046/j.1365-2958.1999.01254.x.View ArticlePubMedGoogle Scholar
- Schesser K, Frithz-Lindsten E, Wolf-Watz H: Delineation and mutational analysis of the Yersinia pseudotuberculosis YopE domains which mediate translocation across bacterial eukaryotic cellular membranes. J Bacteriol. 1996, 178: 7227-7233.PubMed CentralPubMedGoogle Scholar
- Sory MP, Boland A, Lambermont I, Cornelis GR: Identification of the YopE and YopH domains required for secretion and internalization into the cytosol of macrophages, using the cyaA gene fusion approach. Proc Natl Acad Sci USA. 1995, 92: 11998-12002.PubMed CentralView ArticlePubMedGoogle Scholar
- Ramamurthi KS, Schneewind O: Yersinia enterocolitica Type III Secretion: Mutational Analysis of the yopQ Secretion Signal. J Bacteriol. 2002, 184: 3321-3328. 10.1128/JB.184.12.3321-3328.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Ramamurthi KS, Schneewind O: Yersinia yopQ mRNA encodes a bipartite type III secretion signal in the first 15 codons. Mol Microbiol. 2003, 50: 1189-1198. 10.1046/j.1365-2958.2003.03772.x.View ArticlePubMedGoogle Scholar
- Lloyd SA, Norman M, Rosqvist R, Wolf-Watz H: Yersinia YopE is targeted for type III secretion by N-terminal, not mRNA, signals. Mol Microbiol. 2001, 39: 520-531. 10.1046/j.1365-2958.2001.02271.x.View ArticlePubMedGoogle Scholar
- Lloyd SA, Sjöström M, Andersson S, Wolf-Watz H: Molecular characterization of type III secretion signals via analysis of synthetic N-terminal amino acid sequences. Mol Microbiol. 2002, 43: 51-59. 10.1046/j.1365-2958.2002.02738.x.View ArticlePubMedGoogle Scholar
- Woestyn S, Sory MP, Boland A, Lequenne O, Cornelis GR: The cytosolic SycE and SycH chaperones of Yersinia protect the region of YopE and YopH involved in translocation across eukaryotic cell membranes. Mol Microbiol. 1996, 20: 1261-1271.View ArticlePubMedGoogle Scholar
- Birtalan SC, Phillips RM, Ghosh P: Three-dimensional secretion signals in chaperone-effector complexes of bacterial pathogenes. Mol Cell. 2002, 9: 971-980. 10.1016/S1097-2765(02)00529-4.View ArticlePubMedGoogle Scholar
- Boyd AP, Lambermont I, Cornelis GR: Competition between the Yops of Yersinia enterocolitica for delivery into eukaryotic cells: role of the SycE chaperone binding domain of YopE. J Bacteriol. 2000, 182: 4811-4821. 10.1128/JB.182.17.4811-4821.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Lee VT, Anderson DM, Schneewind O: Targeting of Yersinia Yop proteins into the cytosol of HeLa cells: one-step translocation of YopE across bacterial and eukaryotic membranes is dependent on SycE chaperone. Mol Microbiol. 1998, 28: 593-601. 10.1046/j.1365-2958.1998.00822.x.View ArticlePubMedGoogle Scholar
- Cheng LW, Anderson DM, Schneewind O: Two independent type III secretion mechanisms for YopE in Yersinia enterocolitica. Mol Microbiol. 1997, 24: 757-765. 10.1046/j.1365-2958.1997.3831750.x.View ArticlePubMedGoogle Scholar
- Trček J, Wilharm G, Jacobi CA, Heesemann J: Yersinia enterocolitica YopQ: strain dependent cytosolic accumulation and post-translational secretion. Microbiology. 2002, 148: 1457-1465.View ArticlePubMedGoogle Scholar
- Eilers M, Schatz G: Binding of a specific ligand inhibits import of a purified precursor protein into mitochondria. Nature. 1986, 322: 228-232. 10.1038/322228a0.View ArticlePubMedGoogle Scholar
- Wienhues U, Becker K, Schleyer M, Guiard B, Tropschug M, Horwich AL, Pfanner N, Neupert W: Protein folding causes an arrest of preprotein translocation into mitochondria in vivo. J Cell Biol. 1991, 115: 1601-1609. 10.1083/jcb.115.6.1601.View ArticlePubMedGoogle Scholar
- Hynds P, Robinson D, Robinson C: The Sec-independent Twin-arginine Translocation System Can Transport Both Tightly Folded and Malfolded Proteins across the Thylakoid Membrane. J Biol Chem. 1998, 273: 34868-34874. 10.1074/jbc.273.52.34868.View ArticlePubMedGoogle Scholar
- Gaume B, Klaus C, Ungermann C, Guiard B, Neupert W, Brunner M: Unfolding of preproteins upon import into mitochondria. EMBO J. 1998, 17: 6497-6507. 10.1093/emboj/17.22.6497.PubMed CentralView ArticlePubMedGoogle Scholar
- Lee VT, Schneewind O: Yop fusions to tightly folded protein domains and their effects on Yersinia enterocolitica type III secretion. J Bacteriol. 2002, 184: 3740-3745. 10.1128/JB.184.13.3740-3745.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Feldman MF, Müller S, Wuest E, Cornelis GR: SycE allows secretion of YopE-DHFR hybrids by the Yersinia enterocolitica type III Ysc system. Mol Microbiol. 2002, 46: 1183-1197. 10.1046/j.1365-2958.2002.03241.x.View ArticlePubMedGoogle Scholar
- Jacobi CA, Roggenkamp A, Rakin A, Zumbihl R, Leitritz L, Heesemann J: In vitro and in vivo expression studies of yopE from Yersinia enterocolitica using the gfp reporter gene. Mol Microbiol. 1998, 30: 865-882. 10.1046/j.1365-2958.1998.01128.x.View ArticlePubMedGoogle Scholar
- Kopytek SJ, Dyer JC, Knapp GS, Hu JC: Resistance to methotrexate due to AcrAB-dependent export from Escherichia coli. Antimicrob Agents Chemother. 2000, 44: 3210-3212. 10.1128/AAC.44.11.3210-3212.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Heesemann J, Algermissen B, Laufs R: Genetically manipulated virulence of Yersinia enterocolitica. Infect Immun. 1984, 46: 105-110.PubMed CentralPubMedGoogle Scholar
- Chang AC, Cohen SN: Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol. 1978, 134: 1141-1156.PubMed CentralPubMedGoogle Scholar
- Ruckdeschel K, Roggenkamp A, Schubert S, Heesemann J: Differential contribution of Yersinia enterocolitica virulence factors to evasion of microbicidal action of neutrophils. Infect Immun. 1996, 64: 724-733.PubMed CentralPubMedGoogle Scholar
- Birtalan S, Gosh P: Structure of the Yersinia type III secretory system chaperone SycE. Nat Struct Biol. 2001, 8: 974-978. 10.1038/nsb1101-974.View ArticlePubMedGoogle Scholar
- Luo Y, Bertero MG, Frey EA, Pfuetzner RA, Wenk MR, Creagh L, Marcus SL, Lim D, Sicheri F, Kay C, Haynes C, Finlay BB, Strynadka NC: Structural and biochemical characterization of the type III secretion chaperones CesT and SigE. Nat Struct Biol. 2001, 8: 1031-1036. 10.1038/nsb717.View ArticlePubMedGoogle Scholar
- Blocker A, Jouihri N, Larquet E, Gounon P, Ebel F, Parsot C, Sansonetti P, Allaoui A: Structure and composition of the Shigella flexneri "needle complex", a part of its type III secreton. Mol Microbiol. 2001, 39: 652-663. 10.1046/j.1365-2958.2001.02200.x.View ArticlePubMedGoogle Scholar
- Kubori T, Matsushima Y, Nakamura D, Uralil J, Lara-Tejero M, Sukhan A, Galan JE, Aizawa SI: Supramolecular structure of the Salmonella typhimurium type III protein secretion system. Science. 1998, 280: 602-605. 10.1126/science.280.5363.602.View ArticlePubMedGoogle Scholar
- Evdokimov AG, Tropea JE, Routzahn KM, Waugh DS: Crystal structure of the Yersinia pestis GTPase activator YopE. Protein Sci. 2002, 11: 401-408. 10.1110/ps.34102.PubMed CentralView ArticlePubMedGoogle Scholar
- Stuckey JA, Schubert HL, Fauman EB, Zhang ZY, Dixon JE, Saper MA: Crystal structure of Yersinia protein tyrosine phosphatase at 2.5 A and the complex with tungstate. Nature. 1994, 370: 571-575. 10.1038/370571a0.View ArticlePubMedGoogle Scholar
- Cody V, Galitsky N, Luft JR, Pangborn W, Rosowsky A, Blakley RL: Comparison of two independent crystal structures of human dihydrofolate reductase ternary complexes reduced with nicotinamide adenine dinucleotide phosphate and the very tight-binding inhibitor PT523. Biochemistry. 1997, 36: 13897-13903. 10.1021/bi971711l.View ArticlePubMedGoogle Scholar
- Vijay-Kumar S, Bugg CE, Cook WJ: Structure of ubiquitin refined at 1.8 A resolution. J Mol Biol. 1987, 194: 531-544. 10.1016/0022-2836(87)90679-6.View ArticlePubMedGoogle Scholar
- Matouschek A: Protein unfolding – an important process in vivo?. Curr Opin Struct Biol. 2003, 13: 98-109. 10.1016/S0959-440X(03)00010-1.View ArticlePubMedGoogle Scholar
- Gorbalenya AE, Koonin EV: Helicases: amino acid sequence comparisons and structure-function relationships. Curr Opin Struct Biol. 1993, 3: 419-429. 10.1016/S0959-440X(05)80116-2.View ArticleGoogle Scholar
- Lupas AN, Martin J: AAA proteins. Curr Opin Struct Biol. 2002, 12: 746-753. 10.1016/S0959-440X(02)00388-3.View ArticlePubMedGoogle Scholar
- Heesemann J, Gross U, Schmidt N, Laufs R: Immunochemical analysis of plasmid-encoded proteins released by enteropathogenic Yersinia sp. grown in calcium-deficient media. Infect Immun. 1986, 54: 561-567.PubMed CentralPubMedGoogle Scholar
- Nilles ML, Williams AW, Skrzypek E, Straley SC: Yersinia pestis LcrV forms a stable complex with LcrG and may have a secretion-related regulatory role in the low-Ca2+ response. J Bacteriol. 1997, 179: 1307-1316.PubMed CentralPubMedGoogle Scholar
- Wilharm G, Neumayer W, Heesemann J: Recombinant Yersinia enterocolitica YscM1 and YscM2: homodimer formation and susceptibility to thrombin cleavage. Protein Expr Purif. 2003, 31: 167-172. 10.1016/S1046-5928(03)00183-9.View ArticlePubMedGoogle Scholar
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