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Figure 3 | BMC Microbiology

Figure 3

From: In vitro adhesion and invasion inhibition of Shigella dysenteriae, Shigella flexneri and Shigella sonnei clinical strains by human milk proteins

Figure 3

Bacterial proteins recognized by sIgA and Lf. (A) Surface and supernatant proteins. Lane 1, stained with coomassie blue. Lane 2 (lanes 2 and 5) and Lf (lanes 3 and 6) bound to supernatant and surface proteins (A) and intracellular proteins (B). Modified Western Blot, after the transference the membrane containing SSP and IP was incubated with P1S-sIgA or P3H-Lf overnight, following incubation with anti-SC or anti-Lf to respective sIgA or Lf. Marked supernatant with surface proteins have 26, 38, 45, 68 kDa and intracellular proteins with 23; 35; 38; 49; 56; 57; 58 kDa bound to sIgA (lanes 2 and 5). Surface proteins with 23; 26; 38 kDa and intracellular proteins with 23; 38 kDa bound to lactoferrin (lanes 3 and 6).

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