Figure 3From: In vitro adhesion and invasion inhibition of Shigella dysenteriae, Shigella flexneri and Shigella sonnei clinical strains by human milk proteinsBacterial proteins recognized by sIgA and Lf. (A) Surface and supernatant proteins. Lane 1, stained with coomassie blue. Lane 2 (lanes 2 and 5) and Lf (lanes 3 and 6) bound to supernatant and surface proteins (A) and intracellular proteins (B). Modified Western Blot, after the transference the membrane containing SSP and IP was incubated with P1S-sIgA or P3H-Lf overnight, following incubation with anti-SC or anti-Lf to respective sIgA or Lf. Marked supernatant with surface proteins have 26, 38, 45, 68 kDa and intracellular proteins with 23; 35; 38; 49; 56; 57; 58 kDa bound to sIgA (lanes 2 and 5). Surface proteins with 23; 26; 38 kDa and intracellular proteins with 23; 38 kDa bound to lactoferrin (lanes 3 and 6).Back to article page