Table 4 Primers used in PCR and RT-PCR experiments performed in this study Primer sequences are shown 5' to 3'. "L" annotates sense, "R" antisense primers. In addition, the proposed gene names, as depicted in Fig. 1, and PCR product sizes are shown (see also Fig. 5).
From: Presence and expression of hydrogenase specific C-terminal endopeptidases in cyanobacteria
Synechocystis PCC 6803 | Primer Sequence | Gene Name | Product Size [bp] |
|---|---|---|---|
Bidirectional hydrogenase | |||
hoxH6803/L | AAT CCC ACG CCC TAA GTT TT | ||
hoxH | 200 | ||
hoxH6803/R | CAC TGA CCA AGC AGA GTG GA | ||
hoxW6803/L | TCA TCG GTT ACG GCA ATA CC | ||
hoxW | 198 | ||
hoxW6803/R | ATG GTT CGT TTG CTG ATT CC | ||
Nostoc punctiforme ATCC 29133/PCC 73102 | |||
Uptake hydrogenase | |||
hupL-L | CTG TTG GGC GGA CAA TGG CCT CA | ||
hupL | 383 | ||
hupL-R | CCA CTT TTC ATA ATC AT | ||
hybD73102/L | CGC AGG GAT GGA AGT AAT GT | ||
hupW | 218 | ||
hybD73102/R | TCA CAT CAT CGG GAA AGT CA | ||
Anabaena PCC 7120 | |||
Uptake hydrogenase | |||
hupL7120/L2 | TAT ATC ACC CGT CGG TAG AG | ||
hupL | 205 | ||
hupL7120/R2 | CCA TGA TGC AGA GGT TAA GT | ||
hybD7120/L | CGC AGG GAT GGA AGT TAT GT | ||
hupW | 209 | ||
hybD7120/R | GCG GAA AAT CAT CTG GAA A | ||
Bidirectional hydrogenase | |||
hoxHF | CCA CTA TGC TCG TTT AAT TGA AAT | ||
hoxH | 355 | ||
hoxHR | GGA ACA ACT TAA ACA GGG GTC AAA | ||
hoxW7120/L2 | ATC TCT TGC AGT CCA TCA AC | ||
hoxW | 200 | ||
hoxW7120/R2 | CGT AGA TGG CTT TGG TTA AG |