Figure 6From: A novel Leishmania infantum nuclear phosphoprotein Lepp12 which stimulates IL1-beta synthesis in THP-1 transfectantsRecombinant protein Lepp12 is phosphorylated by purified exogenous PKC (ePKC) in the presence of 10 microCi gamma-P32 ATP in an in vitro assay. rLepp12: GST-Lepp12; h: histone mix as positive phosphorylation reaction control; GST; rb: reaction buffer; cl: E. coli lysate, as negative controls. Autoradiogram is shown after exposition time of 30 min.Back to article page