Figure 13

Intracellular Lepp12 activates THP-1 cells to produce IL-1beta and induces an enhancing effect on PMA-stimulated IL-1beta synthesis. IL-1beta was measured as described in FIG. 11 from THP-1 cells (7.5 × 10⁴ cells per 200 microL), untransfected (dotted bars) or GST-transfected (open bars) or GST-Lepp12-transfected (hatched bars), cultured in the absence or in the presence of PMA at indicated concentrations (in ng/ml). GST-Lepp12 protein and GST protein were transfected into the THP-1 cells using Chariot Transfection Kit as described in Methods section. One representative experiment out of three performed is shown