Figure 10
Enzymatic activity present in THP-1 cells phosphorylates natural protein Lepp12, in the presence of 10 microCi gamma-P32 ATP in an in vitro assay. THP-1 lysate (THP-1L) was used as source of enzymatic activity. Natural Lepp12 (nLepp12, lanes 4, 5) was immunoprecipitated from the promastigote nuclear extracts (corresponding to 3 × 109 cells) using anti-Lepp12 IgG as described in FIG. 8. Control immunoprecipitation (cAb) was performed with irrelevant IgG (cAb, lanes 2, 3). Histone mix is also phosphorylated by an enzymatic activity present in THP-1 cells (h, lane 6). In this experiment, a phosphorylated monomeric form in the histone mix was detected, in contrast to the experiment depicted in fig. 9. Autoradiogram is shown after exposition time of 24 h.