|  | phenol/chloroform | phenol/chloroform +CTAB | DNeasy Tissue kit | Easy-DNA kit | Magna Pure | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 | Input copies /μl | Mean output copies/μl | SD of Mean | % Rec. | SD of % Rec. | Mean output copies/μl | SD of Mean | % Rec. | SD of % Rec. | Mean output copies/μl | SD of Mean | % Rec. | SD of % Rec. | Mean output copies/μl | SD of Mean | % Rec. | SD of % Rec. | Mean output copies/μl | SD of Mean | % Rec. | SD of % Rec. |
Pure C. pneumoniae DNA samples | 0 | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - |
 | 10 | 1.79 | 0.213 | 17.9 | 2.1 | 0 | 0 | 0.0 | 0 | 8.08 | 1.21 | 80.8 | 12.1 | 0.661 | 0.520 | 6.6 | 5.2 | 4.60 | 0.377 | 46.0 | 3.8 |
 | 103 | 296 | 15.4 | 29.6 | 1.5 | 11.8 | 3.20 | 1.2 | 0.3 | 658 | 77.7 | 65.8 | 7.8 | 48.9 | 14.5 | 4.9 | 1.4 | 508 | 49.0 | 50.8 | 4.9 |
 | 105 | 2.09 × 104 | 2.55 × 103 | 20.9 | 2.5 | 728 | 107 | 0.7 | 0.1 | 7.54 × 104 | 1.83 × 104 | 75.4 | 18.4 | 3.10 × 103 | 1.83 × 103 | 3.1 | 1.8 | 5.58 × 104 | 7.54 × 103 | 55.8 | 7.5 |
 | av rec % | - | - | 22.8 | 5.7 | - | - | 0.6 | 0.6 | - | - | 74.0 | 12.4 | - | - | 4.9 | 3.0 | - | - | 50.9 | 6.2 |
Aorta tissue samples spiked with C. pneumoniae DNA | 0 | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | - | - | 0 | 0 | -. | - |
 | 10 | 0 | 0 | 0.0 | 0 | 1.31 | 0.240 | 13.1 | 2.4 | 4.56 | 4.19 | 45.6 | 42.0 | 6.34 | 1.50 | 63.4 | 15.0 | 1.83 | 0.622 | 18.3 | 6.2 |
 | 103 | 70.8 | 32.7 | 7.1 | 3.3 | 13.0 | 6.46 | 1.3 | 0.6 | 285 | 23.9 | 28.5 | 2.4 | 255 | 13.7 | 25.5 | 1.4 | 82.0 | 6.08 | 8.2 | 0.6 |
 | 105 | 5.00 × 103 | 2.55 × 103 | 5.0 | 2.4 | 1.40 × 103 | 611 | 1.4 | 1.0 | 2.37 × 104 | 502 | 23.7 | 0.5 | 3.30 × 104 | 7.53 × 103 | 33.0 | 7.5 | 1.61 × 104 | 1.06 × 104 | 16.1 | 10.6 |
 | av rec % | - | - | 4.0 | 3.7 | - | - | 5.3 | 6.2 | - | - | 32.6 | 21.4 | - | - | 40.6 | 19.4 | - | - | 14.2 | 7.3 |
 | over-all avrec % | - | - | 13.4 | 10.8 | - | - | 3.0 | 4.8 | - | - | 53.3 | 27.3 | - | - | 22.8 | 22.9 | - | - | 32.5 | 20.2 |