Figure 2

Two-dimensional gel electrophoresis patterns of proteins synthesized in E. coli exposed to LVFX. Bacterial cells were exposed to 10 μg/ml of LVFX for 15 min and then pulse-labeled for 1 min. A portion of the protein samples from untreated cells (A) or LVFX-treated cells (B) was subjected to two-dimensional gel electrophoresis using 10 % SDS-polyacrylamide gel as the second dimension. Spots enclosed in circles represent the proteins induced during exposure to LVFX. The proteins numbered are interpreted in the text.