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Table 2 The inhibition of methionine adenosyltransferase by purine analogues. The M. smegmatis enzyme was incubated with 1 mM ATP, 10 mM methionine, 20 mM Mg2+, 150 mM K+, and 10 mM inhibitor, as described in the Methods section.

From: Characterisation of methionine adenosyltransferase from Mycobacterium smegmatis and M. tuberculosis

Inhibitor

Inhibition (%)

Inhibitor

Inhibition (%)

8-Chlorotheophylline

7.0 ± 4.1

1,3,7-Trimethyluric acid

0.0 ± 0.0

7-Hydroxypropyl theophylline

16.1 ± 4.0

6-Bromopurine

31.4 ± 1.5

Uric acid

45.6 ± 11.9

7-Methylxanthine

36.3 ± 4.2

Xanthine

35.4 ± 2.5

1-Methylxanthine

35.9 ± 5.8

8-Azaguanine

81.7 ± 2.8

2-Hydroxypurine

33.8 ± 0.6

3,7-Dimethyluric acid

27.9 ± 1.6

6-Chloropurine

31.4 ± 7.3

2,6-Dichloropurine

35.5 ± 6.9

2-Amino-6-chloropurine-9-acetic acid

23.5 ± 8.4

6-Mercaptopurine

40.1 ± 2.3

6-Benzyloxypurine

17.7 ± 3.4

1-Methyluric acid

43.4 ± 2.9

2-Aminopurine

11.0 ± 2.1

Purine riboside

44.4 ± 5.9

6-Methylpurine

0.0 ± 0.0

O-Methylguanine

60.3 ± 5.3

7-Methyluric acid

11.4 ± 2.2

2,6-Diaminopurine

29.3 ± 7.9

6-Cyanopurine

24.2 ± 4.6

2-Amino-6-carboxy ethyl mercaptopurine

31.9 ± 9.8

2-Amino-6-chloropurine riboside

17.2 ± 3.3

6-Propoxypurine

27.9 ± 4.0

6-Dimethyl aminopurine

28.0 ± 5.3

6-Dimethylallyl aminopurine riboside

41.6 ± 5.5

Azathioprine

75.5 ± 5.4

8-Aza-2,6-diaminopurine

40.0 ± 2.2

6-Mercaptopurine riboside

30.0 ± 5.7

6-Chloropurine riboside

17.1 ± 3.5

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