Figure 1

Amplification strategy and organization of the M. paratuberculosis chromosomal origin of replication. The locations of primer pairs used for amplification and sequencing are marked with facing arrows above the kilobase (kb) scale. The GC skew is shown beneath the kb scale and has a window size of 500. OriC, right at the point of the GC inflection, designates the origin of replication. An open reading frame map of the ~11 kb fragment is represented by shaded boxes and the two divergent arrows immediately above identify the direction of transcription. The degree of substitution in comparison to the corresponding M. avium gene is indicated below the gene name. π (tau) is the overall substitution rate, ds is the synonymous substitution rate, and dn is the non-synonymous substitution rate. GidB, glucose inhibited division protein B. RnpA, RNAse protein component A. RpmH, ribosomal protein L34. DnaA, replication initiator. DnaN, DNA polymerase subunit III. GyrB, DNA gyrase subunit B.