Figure 5

Immunofluorescence microscopy of C. pneumoniae infected HEp-2 cells Immunofluorescence microscopy pictures of infected HEp-2 cells fixed in methanol or 3.7% formaldehyde at 72 hours post infection (hpi). Bound antibody was detected with a FITCH conjugated secondary antibody. Fixed cells reacted with 1: Monoclonal antibody recognizing DnaK (MAb18.1), 2: pAb116-rMOMP, 3: pAb110-rOmp2, 4: pAb237-rPmp21, 5: pAb234-rPmp19. 6: Competition IMF. Reaction with pAb201-rPmp8 in the presence of: rPmp8: rPmp8, to which the antibody was raised, rPmp10: rPmp10, to which the antibody cross reacted in IMB, control: no recombinant proteins. As rPmp8 prevents reaction of pAb201-rPmp8 whereas rPmp10 does not affect the reaction, we conclude that the observed reaction is specific for Pmp8.