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Figure 3 | BMC Microbiology

Figure 3

From: Mycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis, is cell wall-associated and expressed during infection of macrophages

Figure 3

Expression of mycosin-1 in mycobacteria in culture and during infection of macrophages. Samples were resolved by SDS-PAGE and analyzed by Western blotting using anti-mycosin-1 antiserum. Lanes: 1, M. smegmatis; 2, M. smegmatis transformed with p19Kpro; 3, M. smegmatis transformed with p19K-P1; 4, M. tuberculosis clinical isolate GSH-3052 cell lysate; 5, M. tuberculosis clinical isolate GSH-3052 culture filtrate after growth in Kirchner's broth for 6 weeks; 6, lysate of uninfected P388D1 macrophages; 7, lysate of M. tuberculosis clinical isolate GSH-3052-infected P388D1 macrophages. Molecular weights (in kDa) are indicated on the left.

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BMC Microbiology

ISSN: 1471-2180

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