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Figure 1 | BMC Microbiology

Figure 1

From: Mycosin-1, a subtilisin-like serine protease of Mycobacterium tuberculosis, is cell wall-associated and expressed during infection of macrophages

Figure 1

Expression of GST-mycosin-1 fusion protein in E. coli . Bacteria were transformed with vector only (pGEX-2T, left panel) or vector expressing GST-mycosin-1 fusion protein (pGEX-P1, right panel). Lysates were prepared before (U) and after (I) induction with 0.1 mM IPTG for 2 hours; recombinant proteins were purified (P) by GST-affinity chromatography. In each case, fractions were resolved by SDS-PAGE (a) and then analyzed by Western blotting (b) using an anti-GST monoclonal antibody as the primary antibody. Molecular weights (in kDa) are indicated on the left.

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