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Table 2 Overview of the different ITS2-fragment lengths observed and of the number of strains identified by means of ITS2-PCR.

From: Identification of cultured isolates of clinically important yeast species using fluorescent fragment length analysis of the amplified internally transcribed rRNA spacer 2 region

Species

Number of strains tested (number used for calculation of the average length)

ITS2-fragment length (bp), calculated by capillary electrophoresis

Previously published lengths (bp)*[9]

Fragment lengths (bp) derived from Genbank sequences

Genbank accession number of sequences used

  

Average

Range

Standard deviation

   

Blastoschizomyces capitatum

1

250.7

  

248

251

AF455443

Candida albicans

116 (19)

281.6

281.0 – 282.6

0.37

279

284

AF217609

C. dubliniensis

1

286.3

   

289

AF218993

C. glabrata

94 (12)

362.6

362.4 – 362.8

0.15

360

365

AF218994

C. guilliermondii

8

323.2

322.7 – 324.2

0.46

321

325

AF218996

C. humicola

1

295.8

     

C. kefyr

7

375.5

375.4 – 375.8

0.17

372

378

AF218997

C. krusei

10 (2)

285.3

285.2 – 285.4

0.13

282

294

L47113

C. lipolytica

3 (2)

180.6

179.9 – 181.1

0.62

 

190

AF218983

C. lusitaniae

9 (4)

200.3

200.2 – 200.4

0.10

199

204

AF218970

C. norvegensis

3 (2)

267.1

266.6 – 267.1

0.62

 

318

AF333096

C. parapsilosis

36 (12)

254.1

253.5 – 255.4

0.60

251

259

U10989

C. rugosa

1

215.0

214.8 – 215.1

0.10

 

223

AF218971

C. tropicalis

34 (28)

270.6

269.3 – 271.2

0.61

269

272 273 274

AF219000 AF219001 AF218992

Cryptococcus albidus

3

353.3

352.4 – 354.7

1.26

350

353

AF218972

C. laurentii ( environmental)

2

272.1

271.9 – 272.3

0.26

 

275

AF218974

C. laurentii (clinical)

1

307.9

  

306

310

AJ421006

C. neoformans var. neoformans

8 (4)

318.3

318.0 – 318.6

0.29

315

320

AF218975

C. neoformans var. gattii

1

320.0

   

321

AF196312

Dekkera bruxellensis

1

261.8

   

263

AF043502

Geotrichum candidum

3 (1)

193.4

  

192

195

AF157596

Malassezia furfur

1

494.4

   

500

AF246896

Saccharomyces cerevisiae

35 (25)

366.6

365.1 – 368.5

0.89

363

365 366 367

AF219005 AF219006 AF219007

Trichosporon asahii

2

301.0

300.8–301.4

0.50

 

304

AB018014

T. asteroides

1

298.6

   

304

AF444416

T. inkin

1

298.7

   

304

AF444420

T. mucoides

1

297.2

   

297

AF444423

T. ovoides

1

297.5

   

303

AF444439

  1. *: Values obtained by using dUTP instead of dTTP for the PCR-based amplification reaction [9].