Table 2 Reference samples analysed with the two PCR assays. * The unit of the numbers is copies/μl and the numbers are the mean of two measurements from two different runs. # The dilution series is serial and four-fold. Dilution no. 1 has the highest concentration and dilution no. 10 has the lowest concentration. Method 1 is the real-time PCR and method 2 is the conventional PCR.