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Table 3 Strains and plasmids used in this studya.

From: Interaction of the Yersinia pestis type III regulatory proteins LcrG and LcrV occurs at a hydrophobic interface

Strain or plasmid

Relevant properties

Source or reference

E. coli

  

   Novablue

recA1 endA1 hsdR17 (rK- mK+) supE44 thi-1 gyrA96 relA1 lac [F' proA+B+lacIqZ ΔM15::Tn10]

Novagen

P. aeruginosa

  

   PAO1

Wild-type strain

K. Young

Y. pestis

  

   KIM8-3002.7

KIM8-3002 ΔlcrG3

[38]

   KIM8-3002.8

KIM8-3002 ΔlcrGV2

[20]

   KIM8-3002.7.MN1

KIM8-3002.7 pCD1::Tn<R6Kγori/KAN-2>

This study

Saccharomyces cerevisiae

  

   Y187

MATa ura3-52 his3-200 ade2-101 trp1-901 leu2-3 112 gal4 Δmet gal80 ΔURA3::GAL1UAS-GAL1TATA-lacZ

Clontech

Plasmidsa

  

   pACT2

GAL4(768–881) AD LEU2 Apr

Clontech

   pACTG

pACT2 + lcrG

[20]

   pAS2-1

GAL4(1–147) DNA-BD TRP1 AprCYHS2

Clontech

   pJM17

pAS2-1 Δcyh2

[20]

   pASV

pAS2-1 + lcrV

[20]

   pJM15

pASV Δcyh2

[20]

   pJM1

pACT2 + lcrG' 1–40b

This study

   pJM2

pACT2 + lcrG' 34–70b

This study

   pJM3

pACT2 + lcrG' 64–95b

This study

   pJM4

pACT2 + lcrG' 1–70b

This study

   pJM5

pACT2 + lcrG' 34–95b

This study

   pJM23

pACT2 + lcrG' 1–33b

This study

   pJM52

pACT2 + lcrG' 7–40b

This study

   pJM53

pACT2 + lcrG' 12–40b

This study

   pBAD18-Kan

araBADp cloning vector, Kmr

[33]

   pAraG18K

pBAD18-Kan + lcrG

[4]

   pAra-HT-V

pBAD24 + His6-LcrV, Apr

[20]

   pJM89

pBAD18-Kan + lcrG (A16R)c

[20]

   pJM99

pBAD18-Kan + lcrG (L30R)c

This study

   pJM131

pBAD18-Kan + lcrG (S23R)c

This study

   pJM110

pACT2 + pcrG

This study

   pJM127

pBAD18 + pcrG

This study

   pJM132

pBAD18-Kan + pcrG

This study

   pJM133

pBAD18 + pcrG (F42L)c

This study

   pJM143

pBAD18-Kan + lcrG (L39R)c

This study

   pJM144

PBAD18-Kan + lcrG (F48R)c

This study

  1. a Numerous plasmids used for two hybrid analysis of LcrG site-directed mutants are not listed here to conserve space. All plasmids are available upon request. b Amino acids of LcrG included in the construct are indicated. c Amino acid substitutions are indicated.