Figure 4

Location of the Fnr and NarL binding sites in the dmsA promoter region. The DNA sequence is numbered relative to the start of transcription. The dmsA Fnr recognition sequence is indicated by the open rectangle. The region of DNA protected by NarL-phosphate from DNase I cleavage on each strand is denoted by the brackets whereas the sequences protected from hydroxyl radical cleavage on each strand are represented by the closed boxes. DNase I hypersensitive sites are marked with asterisks. The RNA polymerase recognition sequences in the -35 and -10 regions are in bold italics. The three consensus NarL binding sites are represented by the solid arrows whereas dashed arrows mark NarL consensus sequences with one mismatch and dotted arrows signify two mismatches.