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Table 3 Strains and plasmids

From: Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei

Strain/plasmid

Description

Reference

B. pseudomallei

  

DD503

Parental strain; polymixin B resistant, zeocin sensitive, kanamycin sensitive (derived from clinical isolate 1026b)

[61]

bpaC KO

Isogenic bpaC mutant strain of DD503; polymixin B resistant, zeocin resistant, kanamycin sensitive

This study

B. mallei

  

ATCC 23344

Wild-type strain; polymixin B resistant, zeocin sensitive, kanamycin sensitive

[75]

bpaC KO

Isogenic bpaC mutant strain of ATCC 23344; polymixin B resistant, zeocin resistant, kanamycin sensitive

This study

E. coli

  

EPI300

Cloning strain

epicentre® Illumina®

TUNERâ„¢

Expression strain for purification of His-tagged BpaC

EMD Millipore

S17

Strain used for conjugational transfer of pKASbpaC.zeo to B. pseudomallei and B. mallei

[76]

pCC1â„¢

Cloning vector, chloramphenicol resistant

epicentre® Illumina®

pCCbpaC

pCC1 containing the B. pseudomallei DD503 bpaC gene, chloramphenicol resistant

This study

pCCbpaC.zeo

pCCbpaC in which a zeocin resistance cassette was introduced near the middle of the bpaC ORF; chloramphenicol and zeocin resistant

This study

pCC1.3

pCC1-based plasmid control, does not confer adherence to human epithelial cells; chloramphenicol resistant

[77]

pKAS46

Mobilizable suicide plasmid; kanamycin resistant

[78]

pKASbpaC.zeo

pKAS46 containing the insert from pCCbpaC.zeo

This study

pEM7ZEO

Source of the zeocin resistance marker

Life Technologiesâ„¢

pELHisBPSL1631-BMA1027

Plasmid expressing aa 392–1068 of B. pseudomallei 1026b BpaC fused to six N-terminal histidine residues, introduced in E. coli TUNER and used to purify His-tagged BpaC protein for antibody production and ELISA experiments; chloramphenicol resistant.

[67]