Strain/plasmid | Description | Reference |
---|---|---|
B. pseudomallei | Â | Â |
DD503 | Parental strain; polymixin B resistant, zeocin sensitive, kanamycin sensitive (derived from clinical isolate 1026b) | [61] |
bpaC KO | Isogenic bpaC mutant strain of DD503; polymixin B resistant, zeocin resistant, kanamycin sensitive | This study |
B. mallei | Â | Â |
ATCC 23344 | Wild-type strain; polymixin B resistant, zeocin sensitive, kanamycin sensitive | [75] |
bpaC KO | Isogenic bpaC mutant strain of ATCC 23344; polymixin B resistant, zeocin resistant, kanamycin sensitive | This study |
E. coli | Â | Â |
EPI300 | Cloning strain | epicentre® Illumina® |
TUNERâ„¢ | Expression strain for purification of His-tagged BpaC | EMD Millipore |
S17 | Strain used for conjugational transfer of pKASbpaC.zeo to B. pseudomallei and B. mallei | [76] |
pCC1™ | Cloning vector, chloramphenicol resistant | epicentre® Illumina® |
pCCbpaC | pCC1 containing the B. pseudomallei DD503 bpaC gene, chloramphenicol resistant | This study |
pCCbpaC.zeo | pCCbpaC in which a zeocin resistance cassette was introduced near the middle of the bpaC ORF; chloramphenicol and zeocin resistant | This study |
pCC1.3 | pCC1-based plasmid control, does not confer adherence to human epithelial cells; chloramphenicol resistant | [77] |
pKAS46 | Mobilizable suicide plasmid; kanamycin resistant | [78] |
pKASbpaC.zeo | pKAS46 containing the insert from pCCbpaC.zeo | This study |
pEM7ZEO | Source of the zeocin resistance marker | Life Technologiesâ„¢ |
pELHisBPSL1631-BMA1027 | Plasmid expressing aa 392–1068 of B. pseudomallei 1026b BpaC fused to six N-terminal histidine residues, introduced in E. coli TUNER and used to purify His-tagged BpaC protein for antibody production and ELISA experiments; chloramphenicol resistant. | [67] |