Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei

Table 1 Characteristics ^a of BMA1027 orthologous genes and their encoded products

Strain^b	Locus tag	Predicted protein (aa)	MW (kDa)	Potential signal sequence cleavage site^c
*B. pseudomallei*
1026b/DD503*	BP1026B_I1575	1,152	107.4	ASA^37▼G, AMA^69▼A
K96243	BPSL1631	1,124	104.8	ASA^37▼G, AMA^69▼A
*B. mallei*
ATCC 23344	BMA1027	1,012	94.7	ASA^37▼G, AMA^69▼A

^aSequence analyses were performed using Vector NTI (Life Technologies™) and online tools available through the ExPASy Bioinformatics Resources Portal.
^bGenomic sequences are available through the NCBI genomic BLAST service.
^cThe putative signal sequence cleavage sites were determined using the SignalP 4.1 server.
^*The B. pseudomallei strain DD503 is a derivative of isolate 1026b in which the AmrAB-OprA antibiotic efflux pump has been deleted to facilitate mutant construction[61]. The BMA1027 orthologs of strains DD503 and 1026b are identical (confirmed by nucleotide sequence analysis, data not shown).

ISSN: 1471-2180