Table 4 The specific primers for PCR amplification
Name | Primer sequence |
|---|---|
fpsssbNcoI | 5′ GGA GGA C CA TGG GGA ACG GAA CGT TAA ATA AAG TCA TG 3′ |
fpsssbHindIII | 5′ TTA AAG CTT TTA AAA AGG CAA ATC ATT TTC TAC AG 3′ |
pcrssbNcoI | 5′ TTA CC A TGG GGC GCG GTG TTA ATA AAG TTA TCA TC 3′ |
pcrssbHindIII | 5′ TTA AAG CTT TCA GAA CGG AAT GTC ATC GTC 3′ |
ptossbNcoI | 5′ GGA GGA CC A TGG CAG GAA CAC TCA ATA AAG TTA TGC 3′ |
ptossbHindIII | 5′ TTA AAG CTT TTA AAA GGG TAG ATC ATC TTC CTC 3′ |
pprssbNcoI | 5′ GGA GGA CC A TGG CCA GTC GTG GTG TAA ATA AGG 3′ |
pprssbBglII | 5′ TTA AGA TCT CTA GAA TGG GAT ATC ATC ATC AAA ATC 3′ |
dpsssbNcoI | 5′ TTA CC A TGG GGA TAA ATA AGG CAA TTT TAA TTG GTA ATC TAG 3′ |
dpsssbHindIII | 5′ TTA AAG CTT CTA GAA GGG TAC GTC GTT AC 3′ |
parssbNcoI | 5′ GGA GGA CC A TGG GGC GCG GTG TTA ATA AAG TTA TCA TC 3′ |
parssbBglII | 5′ TTA AGA TCT CTA GAA AGG AAT GTC ATC GTC 3′ |
pinssbNcoI | 5′ TTA CC A TGG GGT TTA ACC GAA GCG TAA ACA AAG TAG 3′ |
pinssbHindIII | 5′ TTA AAG CTT CTA AAA AGG AAT ATC ATC ATC GAA ATC 3′ |