Skip to main content
Figure 6 | BMC Microbiology

Figure 6

From: Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, and Photobacterium profundum

Figure 6

The half-lives of the SSB proteins. A fixed quantity of each SSB protein was incubated at temperatures ranging from 60°C to 100°C for 0, 1, 2.5, 5, 10, 15, 30, 45, and 60 min. 0.05 pmol 5′-end fluorescein-labelled oligonucleotide (dT)35 was then added. The protein-DNA complexes were separated from the free DNA by 2% agarose gel electrophoresis. The incubation periods for each temperature, where 50% of (dT)35 was bound, were noted.

Back to article page