Figure 3From: Characterization of single-stranded DNA-binding proteins from the psychrophilic bacteria Desulfotalea psychrophila, Flavobacterium psychrophilum, Psychrobacter arcticus, Psychrobacter cryohalolentis, Psychromonas ingrahamii, Psychroflexus torquis, and Photobacterium profundum Binding of SSB proteins to oligo (dT). Fixed quantities (10 pmol) of 5′-end fluorescein-labelled oligonucleotides (dT)35, (dT)76 and (dT)120 were incubated with 50, 100 and 200 pmol of the SSB proteins in 20 μl reaction mixtures for 10 min at 25°C. Symbols I, II and III describe SSB:dT complexes.Back to article page