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Figure 1 | BMC Microbiology

Figure 1

From: Comparison of three tannases cloned from closely related lactobacillus species: L. Plantarum, L. Paraplantarum, and L. Pentosus

Figure 1

Neighbor-joining phylogenetic consensus tree based on amino acid sequences of TanLpl, TanLpa, and TanLpe. The deduced amino acid sequences of TanLpl, TanLpa, and TanLpe were aligned by the ClustalW method using the MEGA5 software package [12]. Phylogenetic trees were constructed using the neighbor-joining method [13] with MEGA5. The percentage of similarity between nucleotide sequences was calculated using BioEdit software [14]. The analysis was based on 469 residues for TanLpl and TanLpa sequences, and 470 residues for TanLpe sequences. The tannase genes of the L. plantarum WCFS1 (GenBank accession no. YP_004890536) and L. pentosus IG1 (GenBank accession no. CCC17686) were used to align with the corresponding genes obtained in this study. The stability of the groupings was estimated by bootstrap analysis with 1,000 replications. The information of used strains and DDBJ accession numbers are listed in Additional file 1: Table S1.

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