High prevalence and two dominant host-specific genotypes of Coxiella burnetii in U.S. milk

BMC Microbiology

Table 3 Genotyping results of Coxiella burnetii DNA from bovine and caprine milk sampled every-other week

Brand ID	Bottling state	Species	Samples	ST20^a	Possible ST20^b	ST8^c	Possible ST8^d	Other STs	Unable to genotype^e
A	Arizona	Bovine	68	67	1	0	0	0	0
B	Arizona	Bovine	68	63	3	0	0	0	2
C	California	Bovine	68	59	6	0	0	0	3
D	Colorado	Bovine	68	65	2	0	0	0	1
E	Idaho	Bovine	68	66	2	0	0	0	0
F	California	Caprine	60	2	0	28	10	2	18
		TOTALS	400	322	14	28	10	2	24

^aSamples genotyped as allele G at locus Cox56bp10. This locus is specific to sequence type 20 where allele G indicates ST20 and allele A excludes ST20.
^bSamples failed to be genotyped with the ST20 specific locus Cox56bp10, likely due to low levels of DNA. However, other loci were used to determine groups of likely sequence types and ST20 was never excluded.
^cSamples genotyped as allele C at locus Cox51bp67. This locus is specific to sequence type 8 where allele C indicates ST8 and allele T excludes ST8.
^dSamples failed to be genotyped with the ST8 specific locus Cox51bp67, likely due to low levels of DNA. However, other loci were used to determine groups of likely sequence types and ST8 was never excluded.
^eAll SNP assays failed to amplify either allele.

ISSN: 1471-2180