Repertoire of novel sequence signatures for the detection of Candidatus Liberibacter asiaticus by quantitative real-time PCR

Kogenaru, Sunitha; Yan, Qing; Riera, Nadia; Roper, M Caroline; Deng, Xiaoling; Ebert, Timothy A; Rogers, Michael; Irey, Michael E; Pietersen, Gerhard; Rush, Charles M; Wang, Nian

doi:10.1186/1471-2180-14-39

Table 2 qRT-PCR detection of Las from plant samples that were collected from different locations in USA and China

From: Repertoire of novel sequence signatures for the detection of Candidatus Liberibacter asiaticus by quantitative real-time PCR

Primer pairs	C_Tvalue of qRT-PCR using infected plant DNA samples as template^#
	DNA samples from Florida, USA												DNA samples from China
	Home stead	Orange	Polk	Lake wales	Highlands	de Soto	St Lucie	Hendry	Hickory	Hardee	Charlotte	Indian river	Hai nan	Jiang xi	Guang xi	Yun nan	Guang dong
P1	23.46	22.24	25.33	22.35	24.72	26.35	23.84	26.00	28.89	26.88	24.71	23.73	27.28	UD	32.55	28.18	UD
P2	24.80	23.10	27.41	23.07	26.90	28.31	25.30	29.27	29.90	29.70	26.99	28.94	28.15	25.69	30.68	28.05	27.67
P3	23.97	22.56	25.03	22.64	24.48	26.06	24.11	25.72	28.62	27.99	24.94	24.31	27.11	UD	34.59	29.95	36.57
P4	24.99	23.03	27.71	23.07	27.12	28.30	25.29	28.49	29.03	27.64	27.46	28.12	28.27	25.77	31.48	27.91	28.03
P5	24.44	22.50	27.40	22.47	26.07	28.17	24.45	28.60	28.91	28.53	26.66	27.69	27.31	25.02	31.68	28.49	26.98
P6	25.49	23.16	28.02	23.26	27.14	29.03	25.27	28.84	29.70	30.08	27.53	28.79	27.68	25.26	33.54	27.79	29.30
P7	24.33	23.01	25.30	22.75	25.31	26.03	24.55	26.55	28.16	28.32	24.87	25.07	27.69	UD	34.71	30.97	UD
P8	23.85	22.73	25.80	22.64	24.62	26.00	23.84	26.20	27.66	26.14	25.58	24.20	27.47	UD	31.19	27.40	UD
P10	24.75	23.76	25.96	23.68	26.05	27.38	25.28	27.85	29.09	28.81	26.11	25.43	28.40	UD	31.74	30.97	UD
P11	25.89	24.02	28.51	24.84	28.55	30.52	26.60	30.52	31.72	30.66	28.08	30.54	28.47	26.09	37.56	35.41	29.28
P16	25.50	23.36	27.87	23.20	26.85	28.41	25.67	29.18	29.41	29.54	27.57	28.88	28.10	25.82	30.54	27.27	27.81
P17	25.95	24.09	28.18	23.65	27.54	29.36	26.61	29.90	29.50	31.09	28.14	30.92	29.34	27.01	36.12	30.28	29.20
P18	25.17	23.11	28.02	23.07	27.43	28.75	25.99	28.96	29.36	29.15	28.19	29.09	28.67	26.41	32.17	27.89	28.79
P23	26.41	24.05	29.28	24.35	28.04	30.22	27.75	31.15	32.14	32.95	29.77	31.48	30.31	27.67	36.73	30.86	30.63
P24	26.14	23.83	28.80	23.68	27.58	29.68	27.28	30.86	32.14	31.87	30.71	31.84	29.75	27.51	37.70	30.80	30.05
P25	25.04	22.68	27.97	22.90	26.67	28.28	25.92	28.63	29.04	30.80	27.30	29.77	27.81	25.47	36.49	29.31	29.31
P26	25.11	23.11	27.65	22.86	27.31	28.53	25.71	28.55	29.57	28.66	27.89	29.49	28.41	26.20	31.67	27.50	28.38
P29	24.73	22.72	27.21	22.60	26.65	27.85	25.42	29.36	29.56	29.28	27.17	29.13	27.39	25.33	34.12	28.03	27.51
P30	26.46	24.87	30.59	24.55	28.91	30.73	27.79	29.69	31.25	31.89	28.33	30.69	29.32	26.60	35.91	29.90	30.71
P31	27.19	25.05	29.83	24.77	29.43	31.03	27.88	31.23	32.67	31.14	29.94	30.71	30.28	27.96	34.28	29.94	31.58
P32	26.65	24.65	29.13	23.73	28.24	29.40	25.93	29.44	30.58	30.20	28.11	29.82	28.94	26.60	33.83	29.23	28.77
P33	25.55	23.35	28.08	23.33	27.03	28.42	26.32	30.32	30.58	30.36	27.83	29.79	28.41	25.80	32.99	30.71	28.37
P34	26.49	24.29	29.62	24.46	28.14	29.45	26.22	28.50	29.66	30.85	26.67	29.28	27.24	25.66	36.14	29.07	29.52
HLBas/r	24.76	22.97	27.55	22.80	31.02	29.94	27.24	27.45	28.02	27.20	28.90	27.95	27.06	25.04	30.40	25.93	25.78

^#Las-infected plant DNA samples were collected from 12 different locations in Florida, USA, and 5 different locations in China. The C_T values indicated are average of three replicates for each primer pairs. UD: Under determined.

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