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Table 1 Bacterial strains used in this study

From: Hyperexpression of α-hemolysin explains enhanced virulence of sequence type 93 community-associated methicillin-resistant Staphylococcus aureus

S. aureus strain

Molecular type

Date of isolation

Place of isolation

Site of isolation

Relevant characteristics

lukSF-PV

reference

Clinical isolates

       

JKD6159

ST93-IV [2B]

2004

Victoria, Australia

Blood

Dominant Australian CA-MRSA clone

+

[14]

TPS3104

ST93-IV [2B]

2009

Western Australia, Australia

Nasal cavity

Dominant Australian CA-MRSA clone

+

This study

TPS3105

ST93-IV [2B]

2005

New South Wales, Australia

Blood

Australian CA-MRSA clone

-

This study

TPS3106

ST93-V [5C2&5]

2008

Western Australia, Australia

Nasal cavity

Australian CA-MRSA clone

-

This study

JKD6272

ST1-IV [2B]

2002

Victoria, Australia

Blood

Australian CA-MRSA clone

-

[14]

JKD6260

ST1-IV [2B]

2008

Western Australia, Australia

Skin

Australian CA-MRSA clone

+

[14]

JKD6177

ST30-IV [2B]

2003

Melbourne, Australia

Blood

Australian CA-MRSA clone

+

[14]

FPR3757 USA300

ST8-IV [2B]

NA

San Francisco, USA

Wrist abscess

Dominant North American CA-MRSA clone

+

[19]

JKD6009

ST239-III [3A]

2002

New Zealand

Wound

Dominant Australian hospital-associated MRSA clone, AUS2/3

-

[20]

Mutant strains

       

JKD6159∆lukSF-PV

ST93-IV [2B]

   

Isogenic unmarked lukSF-PV KO of JKD6159

-

This study

JKD6159∆hla

ST93-IV [2B]

   

Isogenic unmarked hla KO of JKD6159. Deletion encompassed genome coordinates 1121291–1120441.

+

This study

JKD6159∆hla r

ST93-IV [2B]

   

Isogenic unmarked hla KO repaired in JKD6159∆hla. Introduction of a novel PstI site within hla.

+

This study

JKD6159∆psmα

ST93-IV [2B]

   

Isogenic unmarked psm-α KO in JKD6159. Deletion encompassed genome coordinates 453364–45378.

+

This study

JKD6159∆psmα r

ST93-IV [2B]

   

Isogenic unmarked psm-α KO repaired in JKD6159∆psm-α. Introduction of a novel SalI site within psm-α

+

This study

JKD6159∆00043

ST93-IV [2B]

   

Isogenic unmarked SAA6159_00043 KO of JKD6159. Deletion encompassed genome coordinates 53156 - 54561

+

This study

JKD6159_AraCr

ST93-IV [2B]

   

Isogenic AraC/XylS regulator repaired in JKD6159

+

This study

TPS3105r

ST93-IV [2B]

   

Isogenic agrA repair of TPS3105

-

This study

  1. KO: knockout, NA: not available.