Figure 4

Proliferation of Legionella cells in co-culture with A. castellanii. (A) Proliferation of serially diluted culturable cells is represented as a function of the initial number of CFU as assessed on the standard medium (BCYE). (B & C) Proliferation of cell suspensions exposed to various concentrations of HOCl based on the initial number of CFU as assessed on the standard medium (BCYE) (B) or the supplemented medium (BCYES) (C). Dark bars represent the initial number of CFU as assessed on the standard medium or the supplemented medium (BCYES). Gray bars represent the number of CFU as assessed on the BCYE medium after co-incubation with axenic culture of A. castellanii. The values reported are means for duplicate samples in three independent experiments. Error bars indicate SD and asterisks values below the detection limit (<0.1 CFU.ml^-1).