A degenerate PCR-based strategy as a means of identifying homologues of aminoglycoside and β-lactam resistance genes in the gut microbiota

Table 1 Primers used for the detection of β-lactamase and aminoglycoside resistant genes

Location	Primer	Sequence 5′-3′	Amplicon Size (bp)	Annealing Temp°C	Source
β-lactamase genes
Bla _TEM	RH605	TTTCGTGTCGCCCTTATTCC	692	60	Bailey et al. (2011) [22]
	RH606	CCGGCTCCAGATTTATCAGC
	Bla_TEMF	TGGGTGCACGAGTGGGTTAC	526	57	Tenover et al. (1994) [23]
	Bla_TEMR	TTATCCGCCTCCATCCAGTC
Bla _ROB	Bla_ROBF	ATCAGCCACACAAGCCACCT	692	62	Tenover et al. (1994) [23]
	Bla_ROBR	GTTTGCGATTTGGTATGCGA
Bla _SHV	Bla_SHVF	CACTCAAGGATGTATTGTG	885	58	Briñas et al. (2002) [24]
	Bla_SHVR	TTAGCGTTGCCAGTGCTCG
Bla _OXA	Bla_OXAF	TTCAAGCCAAAGGCACGATAG	702	64	Briñas et al. (2002) [24]
	Bla_OXAR	TCCGAGTTGACTGCCGGGTTG
Bla _CTX-M	Bla_CTX-MF	CGTTGTAAAACGACGGCCAGTGAATGTGCAGYACCAGTAARGTKATGGC	600	55	Monstein et al. (2009) [25]
	Bla_CTX-MR	TGGGTRAARTARGTSACCAGAAYCAGCGG
AG resistant genes
aac (3)-I	Faac3-1	TTCATCGCGCTTGCTGCYTTYGA	239	58	Heuer et al. (2002) [20]
	Raac3-1	GCCACTGCGGGATCGTCRCCRTA
aac (3)-II/VI	Faac3-2	GCGCACCCCGATGCMTCSATGG	189	58
	Raac3-2	GGCAACGGCCTCGGCGTARTGSA
	Facc3-6	GCCCATCCCGACGCATCSATGG
	Raac3-6	CGCCACCGCTTCGGCATARTGSA
aac (6′)-II/Ib	Faac6	CACAGTCGTACGTTGCKCTBGG	235	58
	Raac6	CCTGCCTTCTCGTAGCAKCGDAT
ant (2′)-I	Fant	TGGGCGATCGATGCACGGCTRG	428	58
	Rant	AAAGCGGCACGCAAGACCTCMAC
aph (2″)-I	Faphc	CCCAAGAGTCAACAAGGTGCAGA	527	55
	Faphd	GGCAATGACTGTATTGCATATGA	572	55
	Raph	GAATCTCCAAAATCRATWATKCC
aac (6′)-Ie-aph (2″)-Ia	aac-aphF	GAGCAATAAGGGCATACCAAAAATC	505	47	De Fatίma Silva Lopes et al. (2003) [26]
	aac-aphR	CCGTGCATTTGTCTTAAAAAACTGG
	aac6-aph2F	CCAAGAGCAATAAGGGCATACC	222	55	Schmitz et al. (1999) [27]
	aac6-aph2R	CACACTATCATAACCATCACCG

ISSN: 1471-2180